Development of media to accelerate the isolation of indigo-reducing bacteria, which are difficult to isolate using conventional media
Development of media to accelerate the isolation of indigo-reducing bacteria, which are difficult to isolate using conventional media
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开发培养基以加速靛蓝还原细菌的分离，而使用传统培养基难以分离靛蓝还原细菌
DOI：
10.1007/s11274-017-2300-z
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发表时间：
2017
影响因子：
4.1
通讯作者：
Yumoto Isao
中科院分区：
文献类型：
--
作者：
Nishita Masatoshi;Hirota Kikue;Matsuyama Hidetoshi;Yumoto Isao
靛蓝还原菌在靛蓝发酵液中进行自然发酵。由于该工艺的随机性，靛蓝发酵液中的成分因制备批次和发酵周期的不同而不同。为了鉴定新的靛蓝还原菌，细菌的分离是必不可少的。然而，靛蓝还原细菌的分离是困难的，因为传统的培养基往往不适合分离这些生长缓慢的细菌，这些细菌的数量也很少。多糖的水解物和植物基础成分的混合物是加速分离靛蓝还原细菌的候选物，这些细菌不能用传统培养基分离。本研究以麦麸水解物和堆肥靛蓝叶（sukumo）为原料，在厌氧培养靛蓝还原菌的选择培养基中作为发酵液的成分。结果表明，利用常规培养基分离专性和非氧代谢兼性厌氧菌比较困难，而利用氧代谢兼性厌氧菌、生长较快的主要菌株相对容易分离。含有sukumohydrolysate的培养基有利于假硬芽孢杆菌相关菌株的分离，而含有麦麸水解物的培养基有利于两栖杆菌的分离。（包括新物种）。用含麦麸水解物的培养基分离出7种（包括2种新种）靛蓝还原菌，用同时含有麦麸和sukumohydrolysates的培养基分离出6种（包括3种新种）靛蓝还原菌。这些新开发的培养基将有助于在靛蓝发酵和类似靛蓝发酵液的环境中分离未知细菌。
Indigo-reducing bacteria perform natural fermentation in indigo fermentation fluid. Owing to the stochastic nature of the process, the constituent in indigo fermentation fluid differ depending on the prepared batch and fermentation period. To identify new indigo-reducing bacteria, isolation of the bacteria is indispensable. However, isolation of indigo-reducing bacteria is difficult because conventional media are often unsuitable to isolate these slow-growing bacteria that also exist in low numbers. Hydrolysates of polysaccharides and mixtures of plant base constituents are candidates to accelerate the isolation of indigo-reducing bacteria that cannot be isolated using conventional media. In this current study, wheat bran hydrolysate and composted indigo leaves (sukumo) were used as ingredients in the fermentation fluid in the selective medium for indigo-reducing bacteria in anaerobic culture. The results suggested that obligate and oxygen-non-metabolizing facultative anaerobes are difficult to isolate using conventional media, whereas oxygen-metabolizing facultative anaerobes, relatively rapid-growing and major bacterial strains are relatively easy to isolate. Media containingsukumohydrolysate facilitated the isolation of novel species ofBacillus pseudofirmus-related strains, whereas media containing wheat bran hydrolysate facilitated the isolation ofAmphibacillusspp. (including new species). Seven species (including two new species) of indigo-reducing bacteria were isolated using wheat bran hydrolysate-containing media, whereas six species (including three new species) of indigo-reducing bacteria were isolated using media containing both wheat bran andsukumohydrolysates. These newly developed culture media will facilitate the isolation of unknown bacteria in indigo fermentation and in environments similar to indigo fermentation fluid.