DNA methylation of imprinted genes in Mexican-American newborn children with prenatal phthalate exposure.
DNA methylation of imprinted genes in Mexican-American newborn children with prenatal phthalate exposure.
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DOI：
10.2217/epi-2017-0178
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发表时间：
2018-06
期刊：
影响因子：
3.8
通讯作者：
G. Tindula;S. Murphy;Carole Grenier;Zhiqing Huang;K. Huen;Maria Escudero-Fung;A. Bradman;B. Eskenazi;C. Hoyo;N. Holland
中科院分区：
文献类型：
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作者：
G. Tindula;S. Murphy;Carole Grenier;Zhiqing Huang;K. Huen;Maria Escudero-Fung;A. Bradman;B. Eskenazi;C. Hoyo;N. Holland
目的：印迹基因以依赖于父母来源的方式表达，对儿童发育至关重要。最近有限的证据表明，产前暴露于邻苯二甲酸盐，无处不在的内分泌干扰物，可以影响他们的表观遗传失调。材料与方法我们通过焦磷酸测序对墨西哥裔美国人队列中296份脐带血DNA样本的9个印迹基因差异甲基化区域的DNA甲基化进行了量化。胎儿接触邻苯二甲酸盐是通过孕妇尿液样本中邻苯二甲酸盐代谢物浓度来估计的。结果印迹基因的几个差异甲基化区域与高分子量邻苯二甲酸盐相关。在MEG3中观察到最一致的、阳性的和错误的显著关联。结论子宫内邻苯二甲酸盐暴露可能影响新生儿印迹基因的甲基化状态。
AIM Imprinted genes exhibit expression in a parent-of-origin-dependent manner and are critical for child development. Recent limited evidence suggests that prenatal exposure to phthalates, ubiquitous endocrine disruptors, can affect their epigenetic dysregulation. MATERIALS & METHODS We quantified DNA methylation of nine imprinted gene differentially methylated regions by pyrosequencing in 296 cord blood DNA samples in a Mexican-American cohort. Fetal exposure was estimated by phthalate metabolite concentrations in maternal urine samples during pregnancy. RESULTS Several differentially methylated regions of imprinted genes were associated with high molecular weight phthalates. The most consistent, positive, and false discovery rate significant associations were observed for MEG3. CONCLUSION Phthalate exposure in utero may affect methylation status of imprinted genes in newborn children.