No evidence of severe acute respiratory syndrome-coronavirus 2 in semen of males recovering from coronavirus disease 2019
No evidence of severe acute respiratory syndrome-coronavirus 2 in semen of males recovering from coronavirus disease 2019
复制标题
2019 年冠状病毒病康复男性精液中没有证据显示严重急性呼吸综合征 - 冠状病毒 2
DOI：
10.1016/j.fertnstert.2020.04.024
复制
发表时间：
2020-06-01
影响因子：
6.7
通讯作者：
Hotaling, James M.
中科院分区：
文献类型：
--
作者：
Pan, Feng;Xiao, Xingyuan;Hotaling, James M.
目的：描述 2019 年冠状病毒病 (COVID-19) 康复患者精液中严重急性呼吸综合征 (SARS)-冠状病毒 2 (CoV-2) 的检测情况，并描述睾丸内血管紧张素转换酶 2 (ACE2) 和跨膜丝氨酸蛋白酶 2 (TMPRSS2) 的表达谱。设计：观察性横断面研究。设置：三级转诊中心。患者：34 名中国成年男性通过咽拭子样本进行确证性定量逆转录聚合酶链反应 (qRT-PCR) 诊断患有 COVID-19。干预措施：无。主要结果指标：通过单份射精精液样本的 qRT-PCR 鉴定 SARS-CoV-2。未评估精液质量。通过先前发布的单细胞转录组数据集探索人类睾丸中 ACE2 和 TMPRSS2 的表达模式。结果：在 COVID-19 确诊前后，六名患者 (19%) 表现出阴囊不适，提示病毒性睾丸炎。自 COVID-19 诊断起中位 31 天（四分位距，29-36 天）后，精液中未检测到严重急性呼吸综合征-CoV-2。单细胞转录组分析显示 ACE2 和 TMPRSS2 表达稀疏，几乎没有重叠的基因表达。结论：在 COVID-19 诊断后 1 个月从 COVID-19 恢复的患者的精液中未检测到严重急性呼吸综合征-CoV-2。根据 ACE2 和 TMPRSS2 的表达，血管紧张素转换酶 2 介导的 SARS-CoV-2 病毒进入靶宿主细胞不太可能发生在人睾丸内。 SARS-CoV-2 对男性生殖功能的长期影响仍不清楚。 (C) 2020 年，美国生殖医学会。
Objective: To describe detection of severe acute respiratory syndrome (SARS)-coronavirus 2 (CoV-2) in seminal fluid of patients recovering from coronavirus disease 2019 (COVID-19) and to describe the expression profile of angiotensin-converting enzyme 2 (ACE2) and Transmembrane Serine Protease 2 (TMPRSS2) within the testicle.Design: Observational, cross-sectional study.Setting: Tertiary referral center.Patient(s): Thirty-four adult Chinese males diagnosed with COVID-19 through confirmatory quantitative reverse transcriptasepolymerase chain reaction (qRT-PCR) from pharyngeal swab samples.Intervention(s): None.Main Outcome Measure(s): Identification of SARS-CoV-2 on qRT-PCR of single ejaculated semen samples. Semen quality was not assessed. Expression patterns of ACE2 and TMPRSS2 in the human testis are explored through previously published single-cell transcriptome datasets.Result(s): Six patients (19%) demonstrated scrotal discomfort suggestive of viral orchitis around the time of COVID-19 confirmation. Severe acute respiratory syndrome-CoV-2 was not detected in semen after a median of 31 days (interquartile range, 29-36 days) from COVID-19 diagnosis. Single-cell transcriptome analysis demonstrates sparse expression of ACE2 and TMPRSS2, with almost no overlapping gene expression.Conclusion(s): Severe acute respiratory syndrome-CoV-2 was not detected in the semen of patients recovering from COVID-19 1 month after COVID-19 diagnosis. Angiotensin-converting enzyme 2-mediated viral entry of SARS-CoV-2 into target host cells is unlikely to occur within the human testicle based on ACE2 and TMPRSS2 expression. The long-term effects of SARS-CoV-2 on male reproductive function remain unknown. (C) 2020 by American Society for Reproductive Medicine.