The biological features and genetic diversity of novel fish rhabdovirus isolates in China
The biological features and genetic diversity of novel fish rhabdovirus isolates in China
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DOI：
10.1007/s00705-017-3416-z
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发表时间：
2017-09-01
影响因子：
2.7
通讯作者：
Su, Jianguo
中科院分区：
文献类型：
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作者：
Fu, Xiaozhe;Lin, Qiang;Su, Jianguo
Rhabdoviridae是一个多样化的负义单链RNA病毒家族，感染哺乳动物，鸟类，爬行动物，鱼类，昆虫和植物。本文报道了从中国病鱼中分离到的6种新型病毒，分别为SCRV-QY、SCRV-SS、SCRV-GM、CmRV-FS、MsRV-SS、OmbRV-JM。病鱼的典型临床症状是出血。通过观察细胞病变效应、RT-PCR和电镜观察，确定了这些分离株在中华鲈鱼脑细胞中的高效繁殖。序列比对和系统发育分析表明，这些分离物均属于chuatsi sininiperca rhabdovirus的单系谱系。
The Rhabdoviridae is a diverse family of negative-sense single-stranded RNA viruses which infects mammals, birds, reptiles, fish, insects and plants. Herein, we reported the isolation and characterization of 6 novel viruses from diseased fish collected from China including SCRV-QY, SCRV-SS, SCRV-GM, CmRV-FS, MsRV-SS, OmbRV-JM. The typical clinical symptom of diseased fish was hemorrhaging. Efficient propagation of these isolates in a Chinese perch brain cell line was determined by means of observation of cytopathic effect, RT-PCR and electron microscopy. Sequence alignment and phylogenetic analysis of the complete G protein sequences revealed that these isolates were clustered into one monophyletic lineage belonging to the species Siniperca chuatsi rhabdovirus.