Increased sensitivity of sensory neurons to tumor necrosis factor α in rats with chronic compression of the lumbar ganglia
Increased sensitivity of sensory neurons to tumor necrosis factor α in rats with chronic compression of the lumbar ganglia
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DOI：
10.1152/jn.2002.88.3.1393
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发表时间：
2002-09-01
影响因子：
2.5
通讯作者：
Zhang, JM
中科院分区：
文献类型：
--
作者：
Liu, B;Li, HQ;Zhang, JM
促炎细胞因子可能使初级感觉神经元敏感并促进周围神经损伤后神经病理性疼痛过程的发展。本研究的目的是确定在长期压缩的 DRG (CCD) 损伤模型中，背根神经节 (DRG) 神经元对外源性肿瘤坏死因子 α (TNF-α) 的反应是否发生改变。来自被梳理的背根微丝的细胞外记录表明，在 DRG 上急性局部应用 TNF-α 15 分钟，会在正常和 CCD 大鼠中引起 C 纤维和 Abeta 纤维反应。然而，与普通光纤相比，CCD 光纤的响应延迟明显更短，峰值放电率更高。来自小型和大型神经元的细胞内记录表明，与正常神经元相比，TNF-α 在 CCD 神经元中诱导更大的去极化和流变碱的更大减少。 CCD损伤后，对TNF-α有反应的小型和大型神经元的比例显着增加。此外，TNF-α 除了提高自发活动神经元的放电率外，还改变了它们的放电模式。然而，TNF-α 在此类神经元中引起的去极化很小（
Proinflammatory cytokines may sensitize primary sensory neurons and facilitate development of neuropathic pain processes after peripheral nerve injury. The goal of this study was to determine whether responses of dorsal root ganglion (DRG) neurons to exogenous tumor necrosis factor alpha (TNF-alpha) are altered in a chronically compressed DRG (CCD) injury model. Extracellular recordings from teased dorsal root microfilaments demonstrated that acute topical application of TNF-alpha to the DRG for 15 min evoked C- and Abeta-fiber responses in both normal and CCD rats. However, the response latency was significantly shorter, and the peak discharge rate was higher, in CCD fibers than in normal fibers. Intracellular recordings from small- and large-sized neurons showed that TNF-alpha induced greater depolarization and greater decrease in rheobase in CCD neurons than in normal neurons. The proportion of both small- and large-sized neurons that were responsive to TNF-alpha increased significantly after CCD injury. Furthermore, TNF-alpha altered the discharge patterns of large, spontaneously active neurons in addition to enhancing their discharge rates. However, the depolarization caused by TNF-alpha in such neurons was minor (