Cellular composition of human glial cultures from adult biopsy brain tissue
Cellular composition of human glial cultures from adult biopsy brain tissue
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DOI：
10.1016/j.jneumeth.2007.07.005
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发表时间：
2007-10-15
影响因子：
3
通讯作者：
Dragunow, Mike
中科院分区：
文献类型：
--
作者：
Gibbons, Hannah A.;Hughes, Stephanie A.;Dragunow, Mike
小胶质细胞和星形胶质细胞在正常人脑功能和神经系统疾病中起重要作用。为了研究它们的生理和病理作用，需要建立来自成人大脑的体外系统。虽然有几个研究小组已经成功地从人脑中培养出细胞，但这些培养物的组成仍然存在争议。使用形态学标准，免疫细胞化学分析和BrdU掺入试验，我们证明存在不良增殖的小胶质细胞和星形胶质细胞的文化来自癫痫活检组织。此外，我们还发现了第三种细胞类型，即纤维连接蛋白和脯氨酰4-羟化酶免疫阳性的成纤维细胞样细胞，它们高度增殖，并在连续传代培养后成为主要的细胞类型。因此，尽管成人脑组织培养物为研究人类神经胶质细胞提供了极好的资源，但在使用这些方法进行研究时，必须仔细考虑其细胞组成。(C)2007 Elsevier B. V.保留所有权利。
Microglia and astrocytes play vital roles in normal human brain function and in neurological disorders. To study their physiological and pathological roles it is desirable to establish in vitro systems that are derived from the adult human brain. Although several groups have successfully cultured cells from the human brain, the composition of these cultures remains controversial. Using morphological criteria, immunocytochemical analysis and a BrdU incorporation assay we demonstrate the presence of poorly proliferative microglia and astrocytes in cultures derived from epilepsy biopsy tissue. In addition, we characterized a third cell type as fibronectin and prolyl 4-hydroxylase immunopositive fibroblast-like cells, which are highly proliferative and become the predominant cell type after Successive sub-culturing. Therefore, although cultures from adult human brain tissue provide an excellent resource for studying human glial cells, careful consideration must be given to their cellular composition when performing studies using these methods. (C) 2007 Elsevier B.V. All rights reserved.