Detection and comparison of reactive oxygen species (ROS) generated by chlorophyllin metal (Fe, Mg and Cu) complexes under ultrasonic and visible-light irradiation
Detection and comparison of reactive oxygen species (ROS) generated by chlorophyllin metal (Fe, Mg and Cu) complexes under ultrasonic and visible-light irradiation
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DOI：
10.1016/j.ultsonch.2010.12.006
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发表时间：
2011-09-01
影响因子：
8.4
通讯作者：
Li, Ying
中科院分区：
文献类型：
--
作者：
Wang, Jun;Guo, Yuwei;Li, Ying
为了探讨声动力学和光动力学反应的机理，分别用超声波（US）和可见光（VL）辐照叶绿素金属（Chl-M（M = Fe，Mg和Cu））配合物，并用氧化萃取光谱法（OES）检测活性氧（ROS）的产生。也就是说，1，5-二苯基卡巴肼（DPCI）被产生的ROS氧化成1，5-二苯基卡巴腙（DPCO），其可以在563 nm波长附近显示各种可见吸收。此外，还对影响活性氧产生的一些因素进行了综述。结果表明，Chl-M和超声波或可见光照射对ROS的产生具有明显的协同效应。随着超声波或可见光照射时间的延长和Chl-M（M = Fe，Mg，Cu）浓度的增加，活性氧的产生量也随之增加。最后，使用几种猝灭剂来确定所产生的ROS的种类。希望本文的研究能为声动力学疗法（SDT）和光动力学疗法（PDT）的机理研究以及Chl-M在肿瘤治疗中的应用提供参考。(C)2011 Elsevier B. V.保留所有权利。
In this paper, in order to examine the mechanisms of sonodynamic and photodynamic reactions, the chlorophyllin metal (Chl-M (M = Fe, Mg and Cu)) complexes were irradiated by ultrasound (US) and visible-light (VL), respectively, and the generation of reactive oxygen species (ROS) were detected by the method of Oxidation-Extraction Spectrometry (OES). That is, the 1,5-diphenyl carbazide (DPCI) is oxidized by the generated ROS into 1,5-diphenyl carbazone (DPCO), which can display a various visible absorption around 563 nm wavelength. Besides, some influence parameters on the generation of ROS were also reviewed. The results demonstrated an apparent synergistic effect of Chl-M and ultrasonic or visible-light irradiation for the generation of ROS. Moreover, the quantities of generated ROS increase with the increase of (ultrasonic or visible-light) irradiation time and Chl-M (M = Fe, Mg and Cu) concentration. Finally, several quenchers were used to determine the kind of the generated ROS. It is wished that this paper might offer some valuable references for the study on the sonodynamic therapy (SDT) and photodynamic therapy (PDT) mechanisms and the application of Chl-M in tumor treatment. (C) 2011 Elsevier B.V. All rights reserved.