Alterations in renal cilium length during transient complete ureteral obstruction in the mouse
Alterations in renal cilium length during transient complete ureteral obstruction in the mouse
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DOI：
10.1111/j.1469-7580.2008.00918.x
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发表时间：
2008-08-01
影响因子：
2.4
通讯作者：
Deane, James A.
中科院分区：
文献类型：
--
作者：
Wang, Leanne;Weidenfeld, Raphael;Deane, James A.
肾纤毛是一种非运动的感觉细胞器，与肾脏上皮表型的控制有关。肾纤毛缺陷对囊性肾病的影响一直是深入研究的主题。然而，人们对这种细胞器在肾损伤和修复过程中的行为知之甚少。在这里，我们研究了单侧输尿管梗阻和输尿管梗阻逆转小鼠模型中肾纤毛的分布和尺寸。单侧输尿管梗阻 10 天后，观察到整个肾单位和肾集合管的肾纤毛长度大约加倍。在输尿管梗阻解除后发生的肾损伤消退过程中观察到纤毛长度正常化。因此，肾纤毛长度的变化似乎是以前未被重视的肾损伤和修复状态的指标。此外，肾损伤后纤毛长度的增加对肾小管和导管修复过程中上皮表型的规范具有影响。
The renal cilium is a non-motile sensory organelle that has been implicated in the control of epithelial phenotype in the kidney. The contribution of renal cilium defects to cystic kidney disease has been the subject of intense study. However, very little is known of the behaviour of this organelle during renal injury and repair. Here we investigate the distribution and dimensions of renal cilia in a mouse model of unilateral ureteral obstruction and reversal of ureteral obstruction. An approximate doubling in the length of renal cilia was observed throughout the nephron and collecting duct of the kidney after 10 days of unilateral ureteral obstruction. A normalization of cilium length was observed during the resolution of renal injury that occurs following the release of ureteral obstruction. Thus variations in the length of the renal cilium appear to be a previously unappreciated indicator of the status of renal injury and repair. Furthermore, increased cilium length following renal injury has implications for the specification of epithelial phenotype during repair of the renal tubule and duct.