Improvement of leptospirosis surveillance in remote Pacific islands using serum spotted on filter paper
Improvement of leptospirosis surveillance in remote Pacific islands using serum spotted on filter paper
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DOI：
10.1016/j.ijid.2013.12.002
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发表时间：
2014-03-01
影响因子：
8.4
通讯作者：
Cao-Lormeau, Van-Mai
中科院分区：
文献类型：
--
作者：
Musso, Didier;Roche, Claudine;Cao-Lormeau, Van-Mai
目的：钩端螺旋体病是太平洋地区一种严重的被忽视的疾病。由于基本上没有敏感和具体的实验室检测，疾病负担和流行病学数据往往不可靠，无法在知情的情况下确定疾病的优先次序和有效控制。我们报告使用滤纸上的血清斑点，以提高监测钩端螺旋体病在偏远和资源有限的settings.Methods：共172急性期血清样本收集疑似登革热患者在雅普州立医院，密克罗尼西亚联邦，被发现在滤纸上，并通过定期邮件发送到路易斯马拉德研究所，法属波利尼西亚。实时荧光PCR协议进行登革热和钩端螺旋体病确认所有samples.Results：共检测到五个钩端螺旋体病感染疑似denge.Conclusions患者之间：本研究证实了使用滤纸作为一种方便的工具，以提高钩端螺旋体病监测能力在偏远地区。新的监测战略，特别是以经常使用这类工具为基础的战略，对于更充分地描述被忽视疾病的流行病学和负担至关重要。(C)2013作者由爱思唯尔有限公司代表国际传染病学会出版。All rights reserved.
Objectives: Leptospirosis is a serious neglected disease in the Pacific. Because sensitive and specific laboratory tests are largely unavailable, the burden of disease and epidemiological data are often unreliable and do not allow informed disease prioritization and efficient control. We report the use of serum spotted on filter paper to improve the surveillance of leptospirosis in remote and resource-limited settings.Methods: A total of 172 acute-phase serum samples collected from patients with suspected dengue at Yap State Hospital, Federated States of Micronesia, were spotted on filter paper and sent by regular mail to the Institut Louis Malarde, French Polynesia. Real-time PCR protocols for dengue and leptospirosis confirmation were performed on all specimens.Results: A total of five leptospirosis infections were detected amongst the patients with suspected dengue.Conclusions: This study confirms the use of filter paper as a convenient tool to improve leptospirosis surveillance capacity in remote areas. New surveillance strategies, notably based on the regular use of this type of tool, are essential to more adequately describe the epidemiology and burden of neglected diseases. (C) 2013 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. All rights reserved.