INFLUENCE OF ENDOGENOUS CATALASE ACTIVITY ON THE SENSITIVITY OF THE ORAL BACTERIUM ACTINOBACILLUS-ACTINOMYCETEMCOMITANS AND THE ORAL HEMOPHILI TO THE BACTERICIDAL PROPERTIES OF HYDROGEN-PEROXIDE
INFLUENCE OF ENDOGENOUS CATALASE ACTIVITY ON THE SENSITIVITY OF THE ORAL BACTERIUM ACTINOBACILLUS-ACTINOMYCETEMCOMITANS AND THE ORAL HEMOPHILI TO THE BACTERICIDAL PROPERTIES OF HYDROGEN-PEROXIDE
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DOI：
10.1016/0003-9969(85)90141-4
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发表时间：
1985-01-01
影响因子：
3
通讯作者：
GENCO, RJ
中科院分区：
文献类型：
--
作者：
MIYASAKI, KT;WILSON, ME;GENCO, RJ
伴放线放线杆菌（Actinobacillusactinomycetemcomitans）和遗传相关的口腔嗜血杆菌（Haemophilussegnis、Haemophilusaprhophilus和Haemophilusparaphrophilus）对过氧化氢（H2 O2）的致死作用表现出一定的敏感性。放线菌共生菌的抗性最强。为了扩展这一信息，通过在37 ℃温育细菌悬浮液1小时来评估对一定范围的H2 O2浓度（10-6-10-3 M）的敏感性。C在H2 O2的存在下，并将悬浮液涂布在巧克力琼脂平板上，以确定产生50%菌落形成单位（LD 50）减少的H2 O2浓度。过氧化氢酶活性定量与克拉克型氧电极，极谱监测形成的溶解氧在细菌悬浮液的超声波后，加入试剂H2 O2。对H2 O2的敏感性与过氧化氢酶活性无关，无论是在完整的细胞或细菌超声。 具体而言，一些细菌菌株与不可检测的过氧化氢酶活性是高度耐H2 O2。微摩尔浓度的叠氮化钠完全抑制细胞相关过氧化氢酶活性，但不影响A.放线菌共生菌H2 O2。因此，A.放线菌共生菌和某些口腔嗜血菌不是H2 O2杀菌作用抗性的重要决定因素。
Actinobacillus actinomycetemcomitans and the genetically-related oral haemophili (Haemophilus segnis, Haemophilus aprhophilus and Haemophilus paraphrophilus) exhibit a range of sensitivities to the lethal effect of hydrogen peroxide (H2O2), A. actinomycetemcomitans being the most resistant. To extend this information, susceptibility to a range of H2O2 concentrations (10-6-10-3 M) was assessed by incubating bacterial suspensions for 1 h at 37.degree. C in the presence of H2O2 and spreading the suspensions on chocolate agar plates to determine the concentration of H2O2 producing a 50 per cent reduction in colony-forming units (LD50). Catalase activity was quantified with a Clark-type oxygen electrode, which polarographically monitored the formation of dissolved oxygen in bacterial suspensions on sonicates following addition of reagent H2O2. Sensitivity to H2O2 did not correlate with catalase activity, either in intact cells or in bacterial sonicates. Specifically, some bacterial strains with undetectable catalase activity were highly resistant to H2O2. Micromolar concentrations of sodium azide which completely inhibited cell-associated catalase activity did not affect the resistance of A. actinomycetemcomitans to H2O2. Thus, the endogenous catalase activity of A. actinomycetemcomitans and certain oral haemophili is not an important determinant of resistance to the bactericidal effects of H2O2.