Fatty Liver Accompanies an Increase in Lactobacillus Species in the Hind Gut of C57BL/6 Mice Fed a High-Fat Diet
Fatty Liver Accompanies an Increase in Lactobacillus Species in the Hind Gut of C57BL/6 Mice Fed a High-Fat Diet
复制标题
DOI：
10.3945/jn.112.172460
复制
发表时间：
2013-05-01
影响因子：
4.2
通讯作者：
Combs, Gerald F., Jr.
中科院分区：
文献类型：
--
作者：
Zeng, Huawei;Liu, Jun;Combs, Gerald F., Jr.
高脂肪（HF）饮食会导致肥胖，并与非酒精性脂肪肝疾病的发展和肠道微生物组的变化有关。为了检验HF喂养增加某些主要后肠细菌和脂肪性肝炎发展的假设，C57 BL/6小鼠被喂食HF（45%能量）或低脂（LF）（10%能量）饮食10周。饲喂期结束时，HF组体重比LF组高34%（P < 0.05）。这些变化与脂滴数量和大小，炎症细胞浸润，诱导型一氧化氮（NO）合酶蛋白浓度在喂食HF饮食的小鼠肝脏中的急剧增加。与脂肪肝表型一致，在喂食HF饮食的小鼠中，血浆瘦素和肿瘤坏死因子-α浓度也升高，表明慢性炎症。12对聚合酶链反应（PCR）引物中的8对通常占主导地位的后肠微生物生态学的细菌物种从粪便DNA样本中产生特异性PCR产物。HF组中来自加氏乳杆菌和/或台湾乳杆菌的DNA的量是LF组中的6900倍。这些细菌中的许多是耐胆汁酸的，并且能够胆汁酸解缀合。由于胆汁酸是肝脏脂质代谢的调节剂，因此肠道L。gasseri和/或L.在该模型中，HF喂养的台湾种细菌可能在脂肪性肝炎的发展中起作用。J.营养143：627-631，2013.
High-fat (HF) diets can produce obesity and have been linked to the development of nonalcoholic fatty liver disease and changes in the gut microbiome. To test the hypothesis that HF feeding increases certain predominant hind gut bacteria and development of steatohepatitis, C57BL/6 mice were fed an HF (45% energy) or low-fat (LF) (10% energy) diet for 10 wk. At the end of the feeding period, body weights in the HF group were 34% greater than those in the LF group (P < 0.05). These changes were associated with dramatic increases in lipid droplet number and size, inflammatory cell infiltration, and inducible nitric oxide (NO) synthase protein concentration in the livers of mice fed the HF diet. Consistent with the fatty liver phenotype, plasma leptin and tumor necrosis factor-alpha concentrations were also elevated in mice fed the HF diet, indicative of chronic inflammation. Eight of 12 pairs of polymerase chain reaction (PCR) primers for bacterial species that typically predominate hind gut microbial ecology generated specific PCR products from the fecal DNA samples. The amount of DNA from Lactobacillus gasseri and/or Lactobacillus taiwanensis in the HF group was 6900-fold greater than that in the LF group. Many of these bacteria are bile acid resistant and are capable of bile acid deconjugation. Because bile acids are regulators of hepatic lipid metabolism, the marked increase of gut L. gasseri and/or L. taiwanensis species bacteria with HF feeding may play a role in development of steatohepatitis in this model. J. Nutr. 143: 627-631, 2013.