Cytokinins Enhance Sugar-Induced Anthocyanin Biosynthesis in Arabidopsis
Cytokinins Enhance Sugar-Induced Anthocyanin Biosynthesis in Arabidopsis
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DOI：
10.1007/s10059-012-0114-2
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发表时间：
2012-07-01
影响因子：
3.8
通讯作者：
Park, Youn-Il
中科院分区：
文献类型：
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作者：
Das, Prasanta Kumar;Shin, Dong Ho;Park, Youn-Il
在高等植物中，花色素苷的合成受光、糖和激素等多种因素的调控，是由许多转录水平的调控因子介导的。在这里，蔗糖和植物激素，细胞分裂素，在光的存在下进行了研究，以阐明细胞分裂素信号级联导致拟南芥幼苗花青素生物合成基因的转录激活。我们发现，细胞分裂素提高花青素含量和转录水平的糖诱导结构基因UDP-葡萄糖：类黄酮3-O-葡萄糖基转移酶（UF 3GT）和调控基因生产花青素色素1（PAP 1）。遗传分析表明，细胞分裂素信号调节糖诱导的花青素的生物合成，通过一个双组分的信号级联涉及的B型反应调节ARR 1，ARR 10和ARR 12在一个冗余的方式。遗传学、生理学和分子生物学研究表明，细胞分裂素的增强作用部分依赖于光敏色素和隐花色素下游组分HY 5，但主要依赖于光合电子传递。两者合计，我们认为，细胞分裂素的行为下游的光合电子传递链中质体醌氧化还原平衡是由糖调制的感光体独立的方式。
In higher plants, the regulation of anthocyanin synthesis by various factors including light, sugars and hormones is mediated by numerous regulatory factors acting at the transcriptional level. Here, the association between sucrose and the plant hormone, cytokinin, in the presence of light was investigated to elucidate cytokinin signaling cascades leading to the transcriptional activation of anthocyanin biosynthesis genes in Arabidopsis seedlings. We showed that cytokinin enhances anthocyanin content and transcript levels of sugar inducible structural gene UDP-glucose: flavonoid 3-O-glucosyl transferase (UF3GT) and regulatory gene PRODUCTION OF ANTHOCYANIN PIGMENT 1 (PAP1). Genetic analysis showed that cytokinin signaling modulates sugar-induced anthocyanin biosynthesis through a two-component signaling cascade involving the type-B response regulators ARR1, ARR10 and ARR12 in a redundant manner. Genetic, physiological and molecular biological approaches demonstrated that cytokinin enhancement is partially dependent on phytochrome and cryptochrome downstream component HY5, but mainly on photosynthetic electron transport. Taken together, we suggest that cytokinin acts down-stream of the photosynthetic electron transport chain in which the plastoquinone redox poise is modulated by sugars in a photoreceptor independent manner.