Identification of candidate secreted factors involved in trigeminal placode induction
Identification of candidate secreted factors involved in trigeminal placode induction
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DOI：
10.1002/dvdy.21325
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发表时间：
2007-10-01
影响因子：
2.5
通讯作者：
Bronner-Fraser, Marianne
中科院分区：
文献类型：
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作者：
McCabe, Kathryn L.;Shiau, Celia E.;Bronner-Fraser, Marianne
颅外胚层基质对头部周围神经系统的正常发育至关重要。然而，许多方面的分子和组织的相互作用参与他们的诱导尚未阐明。三叉基因由一种来自背神经管的未知分泌因子诱导。为了确定可能参与这一诱导过程的候选基因，我们进行了逆转录聚合酶链反应（RT-PCR）和全负载原位杂交，以筛选未经诱导的三叉神经水平外胚层表达的受体。我们发现成纤维细胞生长因子、胰岛素样生长因子、血小板衍生生长因子、Sonic hedgehog、转化生长因子- β超家族和wnt的受体的表达模式都与三叉基因基板形成的作用一致。这种在三叉神经外胚层中表达的候选受体的RT-PCR筛选是第一个系统筛选，以确定诱发三叉神经基因位的潜在相互作用，是理解这一复杂过程的关键一步。
Cranial ectodermal placodes are critical for normal development of the peripheral nervous system of the head. However, many aspects of the molecular and tissue interactions involved in their induction have yet to be elucidated. The trigeminal placode is induced by an unidentified secreted factor(s) from the dorsal neural tube. To determine candidates that may be involved in this induction process, we have performed reverse transcriptase-polymerase chain reaction (RT-PCR) and whole-mount in situ hybridization to screen for receptors expressed by uninduced presumptive trigeminal level ectoderm. We have found that receptors for fibroblast growth factors, insulin-like growth factors, platelet-derived growth factors, Sonic hedgehog, the transforming growth factor-beta superfamily, and Wnts all are expressed in patterns consistent with a role in trigeminal placode formation. This RT-PCR screen for candidate receptors expressed in presumptive trigeminal ectoderm is the first systematic screen to identify potential interactions underlying induction of the trigeminal placode and represents a critical step for understanding this complex process.