Promotion of hair follicle development and trichogenesis by Wnt-10b in cultured embryonic skin and in reconstituted skin
Promotion of hair follicle development and trichogenesis by Wnt-10b in cultured embryonic skin and in reconstituted skin
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DOI：
10.1016/j.bbrc.2006.04.142
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发表时间：
2006-06-30
影响因子：
3.1
通讯作者：
Ishizaka, S
中科院分区：
文献类型：
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作者：
Ouji, Y;Yoshikawa, M;Ishizaka, S
我们先前表明，Wnt-10 b促进原代皮肤上皮细胞（MPSEC）向毛干和毛囊内根鞘（IRS）细胞在体外的分化。在本研究中，我们发现Wnt-10 b促进毛囊的发展，使用小鼠胚胎皮肤组织的培养和再生，使用裸鼠的重建实验。在用重组Wnt-10 b（rWnt-10 b）培养2天后，在取自胚胎10.5天的小鼠胚胎的皮肤中观察到毛囊发育，然而，没有rWnt-10 b则没有。在Balb/c裸鼠的重建皮肤部位观察到棕色毛发生长，其中将来自C3 H/HeN新生小鼠的真皮成纤维细胞和角质形成细胞移植有产生Wnt-10 b的COS细胞（Wnt-COS）。在没有Wnt-COS共移植的情况下，没有观察到毛发生长。我们的研究结果表明，Wnt-10 b在毛囊发育的启动和毛囊再生中起重要作用。(c)2006年爱思唯尔公司All rights reserved.
We previously showed that Wnt-10b promoted the differentiation of primary skin epithelial cells (MPSEC) toward hair shaft and inner root sheath of the hair follicle (IRS) cells in vitro. In the present study, we found that Wnt-10b promotes the development of hair follicles using a culture Of Mouse embryonic skin tissue and trichogenesis using a reconstitution experiment with nude mice. Hair follicle development was observed in skin taken from mouse embryos on embryonic day 10.5 following a 2-day Culture with recombinant Wnt-10b (rWnt-10b), however, not without rWnt-10b. Brown hair growth was observed at the site of reconstituted skin in Balb/c nude mice where dermal fibroblasts and keratinocytes, derived from C3H/HeN new born mice, were transplanted with Wnt-10b-producing COS cells (Wnt-COS). Without the co-transplantation of Wnt-COS, no hair growth was observed. Our results suggest an important role of Wnt-10b in the initiation of hair follicle development and following trichogenesis. (c) 2006 Elsevier Inc. All rights reserved.