Nuclear patterns of human breast cancer cells during apoptosis: characterisation by fractal dimension and co-occurrence matrix statistics
Nuclear patterns of human breast cancer cells during apoptosis: characterisation by fractal dimension and co-occurrence matrix statistics
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DOI：
10.1007/s00441-005-0030-2
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发表时间：
2005-11-01
影响因子：
3.6
通讯作者：
Castelli, C
中科院分区：
文献类型：
--
作者：
Losa, GA;Castelli, C
采用分形几何和灰度共生矩阵（GLCM）统计相结合的方法，研究了雌激素不敏感的SK-BR 3人乳腺癌细胞凋亡过程中超微结构的变化。细胞凋亡诱导1 μ M的钙霉素（A23187钙离子载体），并通过测量传统的细胞参数在培养期间进行评估。SK-BR 3细胞进入凋亡的早期阶段，在24小时内治疗与卡西霉素，诱导可检测到的变化，核成分，记录的大多数GLCM参数值的增加和分形维数的普遍减少。在这些受影响的细胞中，形态特征伴随着不同的神经节苷脂的减少和细胞表面无法识别的糖脂分子的丢失。所有这些变化都被证明是参与细胞凋亡的检测之前，传统的标志物，这是唯一可测量的凋亡细胞死亡的活跃阶段。在明显的凋亡细胞与1 μ M的卡西霉素处理72小时，大多数核组件经历了戏剧性的超微结构变化，包括边缘化和染色质的凝聚，反映在一个显着减少其分形维数。因此，分形和GLCM分析证实，细胞核的形态重组，归因于结构复杂性的损失，发生在细胞凋亡的早期。
An analytical strategy combining fractal geometry and grey-level co-occurrence matrix (GLCM) statistics was devised to investigate ultrastructural changes in oestrogen-insensitive SK-BR3 human breast cancer cells undergoing apoptosis in vitro. Apoptosis was induced by 1 mu M calcimycin (A23187 Ca2+ ionophore) and assessed by measuring conventional cellular parameters during the culture period. SK-BR3 cells entered the early stage of apoptosis within 24 h of treatment with calcimycin, which induced detectable changes in nuclear components, as documented by increased values of most GLCM parameters and by the general reduction of the fractal dimensions. In these affected cells, morphonuclear traits were accompanied by the reduction of distinct gangliosides and loss of unidentifiable glycolipid molecules at the cell surface. All these changes were shown to be involved in apoptosis before the detection of conventional markers, which were only measurable during the active phases of apoptotic cell death. In overtly apoptotic cells treated with 1 mu M calcimycin for 72 h, most nuclear components underwent dramatic ultrastructural changes, including marginalisation and condensation of chromatin, as reflected in a significant reduction of their fractal dimensions. Hence, both fractal and GLCM analyses confirm that the morphological reorganisation of nuclei, attributable to a loss of structural complexity, occurs early in apoptosis.