inscuteable and numb mediate asymmetric muscle progenitor cell divisions during Drosophila myogenesis
inscuteable and numb mediate asymmetric muscle progenitor cell divisions during Drosophila myogenesis
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DOI：
10.1101/gad.12.3.304
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发表时间：
1998-02-01
影响因子：
10.5
通讯作者：
Chia, W
中科院分区：
文献类型：
--
作者：
Carmena, A;Murugasu-Oei, B;Chia, W
每个幼虫半体节包括类似于30独特指定的躯体肌肉。这些来源于肌肉创始人，这些创始人作为肌肉祖细胞分裂的不同兄弟对出现。我们分析了三个中胚层谱系的祖细胞分裂，产生肌肉（和心包细胞）的创始人。我们的研究结果表明，Insuteable和Numb蛋白定位为分裂祖细胞相对两侧的皮质新月。Numb不对称分离到其中一个兄弟姐妹成肌细胞中取决于inscuteable，并且对于不同兄弟姐妹细胞命运的规范至关重要。失去numb或inscuteable导致相反的细胞命运的转变，都阻止同胞成肌细胞采取不同的身份，导致复制或删除中胚层结构。我们的研究结果表明，肌肉祖细胞分裂本质上是不对称的，此外，参与的inscuteable和numb/N表明，规格的兄弟姐妹成肌细胞的不同细胞的命运需要内在和外在的线索。
Each larval hemisegment comprises similar to 30 uniquely specified somatic muscles. These derive from muscle founders that arise as distinct sibling pairs from the division of muscle progenitor cells. We have analyzed the progenitor cell divisions of three mesodermal lineages that generate muscle (and pericardial cell) founders. Our results show that Inscuteable and Numb proteins are localized as cortical crescents on opposite sides of dividing progenitor cells. Asymmetric segregation of Numb into one of the sibling myoblasts depends on inscuteable and is essential for the specification of distinct sibling cell fates. Loss of numb or inscuteable results in opposite cell fate transformations-both prevent sibling myoblasts from adopting distinct identities, resulting in duplicated or deleted mesodermal structures. Our results indicate that the muscle progenitor cell divisions are intrinsically asymmetric; moreover, the involvement of both inscuteable and numb/N suggests that the specification of the distinct cell fates of sibling myoblasts requires intrinsic and extrinsic cues.