Genetic algorithm-based medium optimization for enhanced production of fluorescent pseudomonad R81 and siderophore
Genetic algorithm-based medium optimization for enhanced production of fluorescent pseudomonad R81 and siderophore
复制标题
DOI：
10.1016/j.bej.2009.07.010
复制
发表时间：
2009-12-01
影响因子：
3.9
通讯作者：
Bisaria, V. S.
中科院分区：
文献类型：
--
作者：
Sarma, M. V. R. K.;Sahai, Vikram;Bisaria, V. S.
荧光假单胞菌R81是一种定殖于植物根部的细菌，具有促进植物生长的特性，是一种很有潜力的生物菌剂。它产生异羟肟酸型铁载体，参与植物的疾病抑制。采用遗传算法（GA）对摇瓶实验中铁载体和细胞批量生产同时进行优化。在80次实验中，共优化了10种培养基组分。在优化的培养基中，铁载体浓度达到1.9g/L，菌体质量浓度达到2.8g/L。CA的应用非常适合于确定双目标函数的培养基成分的最佳浓度水平。与基于RSM的优化相比，CA能够将铁载体浓度增加2.8倍。此外，在没有pH控制的14 L生物反应器中，GA优化的培养基的分批发酵在36 h内产生2.2 g/L铁载体，是迄今为止报道的最高值。CA也被成功地用于分批发酵数学模型的动力学参数估计。(C)2009 Elsevier B. V.保留所有权利。
Fluorescent pseudomonad R81, a root-colonizing bacterium, is a potential bio-inoculant due to its plant growth promoting characteristics. It produces hydroxamate-type siderophore which is involved in disease suppression in plants. Genetic algorithm (GA) methodology was applied for the optimization of siderophore and cell mass production simultaneously in shake flask experiments. A total of 10 medium components were optimized within 80 experiments. A high siderophore concentration of 1.9 g/L and cell mass concentration of 2.8 g/L was achieved in the optimized medium. The application of CA was well suited for determination of optimum concentration levels of the medium constituents for a bi-objective function. CA was able to increase the siderophore concentration by 2.8-fold when compared to RSM-based optimization. Further, the batch fermentation of the GA-optimized medium in 14 L bioreactor without pH control produced 2.2 g/L siderophore in 36 h, the highest reported so far. CA was also successfully used to estimate the kinetic parameters of the mathematical models of the batch fermentation. (C) 2009 Elsevier B.V. All rights reserved.