Subsets of leg proprioceptors influence leg kinematics but not interleg coordination in Drosophila melanogaster walking
Subsets of leg proprioceptors influence leg kinematics but not interleg coordination in Drosophila melanogaster walking
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DOI：
10.1242/jeb.244245
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发表时间：
2022-10-01
影响因子：
2.8
通讯作者：
Bueschges, Ansgar
中科院分区：
文献类型：
--
作者：
Chockley, Alexander S.;Dinges, Gesa F.;Bueschges, Ansgar
陆生动物的腿部运动通常是交配和生存所必需的，运动行为必须是健壮和适应性强的。这种适应性很大程度上是由本体感受器提供的，本体感受器监测身体部位的位置和运动，并向运动网络的其他组件提供反馈。在昆虫中，本体感受弦音器官跨越关节并编码节段之间的相对运动参数。以前的研究已经使用了全器官消融，减少准备或广泛的生理操作来损害股弦音器官（fCO）的功能，该器官监测股骨-胫骨关节，并已证明其对腿间协调和行走行为的贡献。果蝇的fCO包括不同的形态和编码特性（俱乐部，钩，爪）的神经元组; fCO功能的亚种群水平的操作还没有方法访问。在这里，我们利用了D.黑腹动物的fCO神经元的亚群进行鉴定，并使用瞬时光遗传学抑制来研究它们在运动协调中的作用。我们的研究结果表明，俱乐部和钩神经元的一个子集的光遗传学抑制复制抑制整个fCO的影响，但是，单独抑制时，个别子集类型并没有强烈影响单腿运动学的空间方面。此外，fCO子集似乎只发挥次要作用，腿间的时间协调。因此，fCO包含功能不同的亚组，并且这种功能分类可能与基于解剖学和编码特性的分类不同;这应该在未来本体感受器及其参与运动网络的研究中进行研究。
Legged locomotion in terrestrial animals is often essential for mating and survival, and locomotor behavior must be robust and adaptable to be successful. This adaptability is largely provided by proprioceptors monitoring positions and movements of body parts and providing feedback to other components of locomotor networks. In insects, proprioceptive chordotonal organs span joints and encode parameters of relative movement between segments. Previous studies have used whole-organ ablation, reduced preparations or broad physiological manipulations to impair the function of the femoral chordotonal organ ( fCO), which monitors the femur-tibia joint, and have demonstrated its contribution to interleg coordination and walking behavior. The fCO in Drosophila melanogaster comprises groups of neurons that differ in their morphology and encoding properties (club, hook, claw); sub-population-level manipulations of fCO function have not been methodologically accessible. Here, we took advantage of the genetic toolkit available in D. melanogaster to identify sub-populations of fCO neurons and used transient optogenetic inhibition to investigate their roles in locomotor coordination. Our findings demonstrate that optogenetic inhibition of a subset of club and hook neurons replicates the effects of inhibiting the whole fCO; when inhibited alone, however, the individual subset types did not strongly affect spatial aspects of single-leg kinematics. Moreover, fCO subsets seem to play only a minor role in interleg temporal coordination. Thus, the fCO contains functionally distinct subgroups, and this functional classification may differ from those based on anatomy and encoding properties; this should be investigated in future studies of proprioceptors and their involvement in locomotor networks.