Possible role of calcium on oocyte development after intracytoplasmic sperm injection in quail (Coturnix japonica).
Possible role of calcium on oocyte development after intracytoplasmic sperm injection in quail (Coturnix japonica).
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DOI：
10.1002/jez.a.418
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发表时间：
2007-11
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影响因子：
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通讯作者：
S. Mizushima;S. Takagi;T. Ono;Y. Atsumi;A. Tsukada;N. Saito;K. Shimada
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文献类型：
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作者：
S. Mizushima;S. Takagi;T. Ono;Y. Atsumi;A. Tsukada;N. Saito;K. Shimada
尽管脊椎动物卵母细胞胞内钙离子浓度的升高对卵母细胞受精或激活的启动起着关键作用，但在鸟类中还没有关于这一主题的研究报道。本研究旨在研究细胞内钙离子在鸡卵母细胞受精过程中的作用。首先，将精子注入鹌鹑卵母细胞后，立即在培养基中用氯化锶作为细胞内钙升高的诱导剂，以0、2.5、5、7.5、10 mM的剂量处理4小时，然后培养20小时。用5 mM氯化锶处理诱导24.2%的注射卵的胚盘发育，尽管没有卵母细胞在没有锶处理的情况下发育。其次，向鹌鹑卵母细胞注射精子和0.1 M氯化钙或精子和盐水溶液，在无钙的情况下培养4小时，然后在无锶的情况下培养20小时。钙溶液诱导胚盘发育的卵母细胞的20.5%，虽然没有卵母细胞没有钙处理。第三，鹌鹑卵母细胞注射1，2-双（邻氨基苯氧基）乙烷-N，N，N ′，N ′-四乙酸（BAPTA）作为钙螯合剂，用锶（5 mM）培养4小时，然后不加锶培养20小时。只有一个卵母细胞后，BAPTA和锶处理的36个卵母细胞检查。所有卵母细胞的发育阶段从IV到VII不等。这些结果表明，细胞内钙升高可能参与鹌鹑卵母细胞激活，并允许受精和胚盘发育。
Although a rise in intracellular calcium concentration of vertebrate oocytes plays a pivotal role for the initiation of fertilization or oocyte activation, no study on this subject has been reported in birds. This study was conducted to study the role of intracellular calcium in relation to fertilization in avian oocytes. First, immediately after a quail oocyte was injected with a sperm, it was treated with strontium chloride as an inducer for intracellular calcium rise at doses of 0, 2.5, 5, 7.5, 10 mM for 4 hr in the culture medium and was followed by 20-hr culture. Treatment with 5 mM of strontium chloride induced blastodermal development in 24.2% of injected eggs, although no oocytes developed without strontium treatment. Second, quail oocytes were injected with a sperm and 0.1 M calcium chloride or a sperm and saline solution, cultured without calcium for 4 hr and was followed by 20-hr culture without strontium. The calcium solution induced blastodermal development in 20.5% of the oocytes, although no oocytes developed without calcium treatment. Third, quail oocytes were injected with 1,2-bis (o-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid (BAPTA) as a calcium chelator, cultured with strontium (5 mM) for 4 hr followed by 20-hr culture without strontium. Only one oocyte developed after BAPTA and strontium treatment of 36 oocytes examined. Developmental stages of all the oocytes ranged from IV to VII. These results suggest that intracellular calcium rise may participate in quail oocyte activation and allow fertilization and blastodermal development.