In vitro recordings of afferent fibres with receptive fields in the serosa, muscle and mucosa of rat colon
In vitro recordings of afferent fibres with receptive fields in the serosa, muscle and mucosa of rat colon
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DOI：
10.1111/j.1469-7793.1999.0271r.x
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发表时间：
1999-07-01
影响因子：
5.5
通讯作者：
Blackshaw, LA
中科院分区：
文献类型：
--
作者：
Lynn, PA;Blackshaw, LA
1.结肠传入纤维记录使用一种新的体外制备。在结肠粘膜中有末端的纤维被描述，沿着那些在肌肉和浆膜中，以及它们对一系列机械和化学管腔刺激的反应。对组织施加机械刺激，包括拉伸、钝性探查粘膜和用von Frey毛发（10-1000 mg）抚摸粘膜。将化学刺激施加到置于纤维的机械感受野上的环中;这些是蒸馏水、154和308 mM NaCl、100 μ M辣椒素、50 mM HCl和未稀释的50%雪貂胆汁。记录从52个纤维，其中12个表现出的特点，有结束在粘膜。粘液传入神经对10 mg von Frey毛发敏感，并且通常对大于或等于1种化学刺激敏感。有10种纤维具有肌层感受野的特征。这些纤维容易对圆周拉伸以及钝性探测作出反应。27根纤维在纤维层中具有末端的特征。它们迅速适应周向拉伸，并对浆膜的钝性探测作出反应。测试的19种Serum纤维中有15种也对管腔化学物质有反应。三种纤维对所有机械刺激都没有反应，但被化学刺激招募。这是第一次使用体外方法表征结肠传入纤维，也是第一次记录传入纤维的末端位于结肠粘膜中。这些纤维可能在结肠感觉和反射控制方面很重要。
1. Colonic afferent fibres were recorded using a novel in vitro preparation. Fibres with endings in the colonic mucosa are described, along with those in muscle and serosa, and their responses to a range of mechanical and chemical luminal stimuli.2. Mechanical stimuli were applied to the tissue, which included stretch, blunt probing of the mucosa and stroking of the mucosa with von Frey hairs (10-1000 mg). Chemical stimuli were applied into a ring that was placed over the mechanoreceptive field of the fibre; these were distilled water, 154 and 308 mM NaCl, 100 mu M capsaicin, 50 mM HCl, and undiluted and 50 % ferret bile.3. Recordings were made from 52 fibres, 12 of which showed characteristics of having endings in the mucosa. Mucosal afferents were sensitive to a 10 mg von Frey hair and were generally chemosensitive to greater than or equal to 1 chemical stimulus.4. Ten fibres showed characteristics of having receptive fields in the muscular layer. These fibres responded readily to circumferential stretch, as well as to blunt probing.5. Twenty-seven fibres showed characteristics of having endings in the serosal layer. They adapted rapidly to circumferential stretch and responded to blunt probing of the serosa. Fifteen of 19 serosal fibres tested also responded to luminal chemicals.6. Three fibres were unresponsive to all mechanical stimuli but were recruited by chemical stimuli.7. This is the first characterization of colonic afferent fibres using an in vitro method and the first documentation of afferent fibres with their endings in the mucosa of the colon. These fibres are likely to be important in aspects of colonic sensation and reflex control.