Immunogenicity of RepliVAX WN, a novel single-cycle West Nile virus vaccine.
Immunogenicity of RepliVAX WN, a novel single-cycle West Nile virus vaccine.
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RepliVAX WN（一种新型单周期西尼罗河病毒疫苗）的免疫原性。
DOI：
10.1016/j.vaccine.2010.10.069
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发表时间：
2010
期刊：
影响因子：
5.5
通讯作者：
Milligan,GreggN
中科院分区：
文献类型：
--
作者：
Nelson,MichelleH;Winkelmann,Evandro;Ma,Yinghong;Xia,Jingya;Mason,PeterW;Bourne,Nigel;Milligan,GreggN
我们最近报道了用单循环西尼罗河病毒（WNV）疫苗<$VAX WN免疫保护小鼠免受WNV攻击。我们通过表征dVAX WN引发的抗体和T细胞应答扩展了这些研究。免疫后8个月检测到主要由IgG 2c和IgG 2b亚类组成的WNV特异性IgG抗体应答。检测到针对结构和非结构WNV蛋白的强烈的WNV特异性CD 4+和CD 8 +T细胞应答，其特征在于细胞溶解活性和IFN-γ和TNF-α的分泌。重要的是，dVAX WN免疫导致在免疫后8个月检测到强烈的CD 8+记忆T细胞应答。
We recently reported that immunization with RepliVAX WN, a single-cycle West Nile virus (WNV) vaccine, protected mice against WNV challenge. We have extended these studies by characterizing the RepliVAX WN-elicited antibody and T cell responses. WNV-specific IgG antibody responses comprised predominantly of IgG2cand IgG2bsubclasses were detected 8 months after immunization. Vigorous WNV-specific CD4+and CD8+T cell responses directed at both structural and nonstructural WNV proteins were detected which were characterized by cytolytic activity and secretion of IFN-γ and TNF-α. Importantly, RepliVAX WN immunization resulted in vigorous CD8+memory T cell responses detected at 8 months after immunization.