Single-Component Pheromone Consisting of Bombykal in a Diurnal Hawk Moth, Neogurelca himachala sangaica
Single-Component Pheromone Consisting of Bombykal in a Diurnal Hawk Moth, Neogurelca himachala sangaica
复制标题
DOI：
10.1007/s10886-016-0714-y
复制
发表时间：
2016-06
影响因子：
2.3
通讯作者：
T. Uehara;Hiroshi Kitahara;Hideshi Naka;S. Matsuyama;T. Ando;H. Honda
中科院分区：
文献类型：
--
作者：
T. Uehara;Hiroshi Kitahara;Hideshi Naka;S. Matsuyama;T. Ando;H. Honda
最近的研究表明，天蛾共享信息素成分，但在它们的信息素混合物的质量和数量的差异性分离。在对其他种类性信息素的野外测定中，经常捕获到一种昼行性天蛾Neogurelca himachala sangaica（鳞翅目：天蛾科），但该种性信息素的组成尚不清楚。分析己烷提取物的信息素腺体的呼叫女性的气相色谱（GC）使用触角电位检测器（EAD）揭示了两个组件，引起EAD反应的雄蛾触角。这些组分通过其质谱和保留指数在两个GC柱上鉴定为（10 E，12 Z）-10，12-十六碳二烯醛（E10，Z12-16：Ald）及其痕量的（10 E，12 E）-异构体（E10，E12-16：Ald），比例为98：2。在田间试验中，E10，Z12-16：Ald单独吸引雄蛾，并添加E10，E12-16：Ald显着降低吸引力，即使在自然发生的比例。用广义线性混合模型分析表明，E10，Z12-16：Ald对引诱力有正效应，而E10，E12-16：Ald对引诱力有负效应。himachala sangaicaconsists solely of E10，Z12-16：Ald，bombykal. E10、E12-16：Ald对引诱力的负效应可能促进了该单组分信息素系统的种特异性。
Recent work has suggested that hawk moths share pheromone components but are sexually separated by qualitative and quantitative differences in their pheromone blends. During field assays on the sex pheromones of other species, a diurnal hawk moth,Neogurelca himachala sangaica(Lepidoptera: Sphingidae), was frequently captured, but the composition of the sex pheromone of this species was not known. Analysis of hexane extracts of the pheromone glands of calling female by gas chromatography (GC) using an electroantennographic detector (EAD) revealed two components that elicited EAD responses from male moth antennae. These components were identified by their mass spectra and retention indices on two GC columns as (10E,12Z)-10,12-hexadecadienal (E10,Z12–16:Ald) and a trace of its (10E,12E)-isomer (E10,E12–16:Ald) in 98:2 ratio. In field experiments, E10,Z12–16:Ald alone attracted male moths, and addition of E10,E12–16:Ald significantly reduced the attractiveness, even at the naturally-occurring ratio. Analysis of the data using a generalized linear mixed model showed that E10,Z12–16:Ald positively contributed to attractiveness, whereas E10,E12–16:Ald did so negatively, and it was concluded that the sex pheromone ofN. himachala sangaicaconsists solely of E10,Z12–16:Ald, bombykal. The negative effect of E10,E12–16:Ald on attractiveness could promote the species-specificity of this single-component pheromone system.