Elucidation of stability profiles of common chemistry analytes in serum stored at six graded temperatures
Elucidation of stability profiles of common chemistry analytes in serum stored at six graded temperatures
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DOI：
10.1515/cclm-2018-1109
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发表时间：
2019-09-01
影响因子：
6.8
通讯作者：
Ichihara, Kiyoshi
中科院分区：
文献类型：
--
作者：
Shimizu, Yoshihisa;Ichihara, Kiyoshi
背景：许多报告涉及血清中生化分析物的稳定性。然而，目前还没有涵盖广泛储存温度范围的研究。使用设备，使精确的温度控制，我们调查了六种不同的存储温度对血清analysts.Methods的效果：从7名健康志愿者的血清标本，并分为多个等份存储在-30，-20，-10，0，4，和25摄氏度。在第1、3、7、14、28和56天，将在各温度下储存的等分试样重新置于维持在-80 ℃的深冻器中。结果：（1）在25 ℃时，丙氨酸氨基转移酶（ALT）、肌酸激酶（CK）、天冬氨酸氨基转移酶（AST）和总胆红素（TBil）均不稳定，尤其是在第7天及以后。(2)在=-20 ℃时。(5)在-30摄氏度，所有分析物的测试结果一般都非常稳定，除了ALT和CK，这表明显着的活动减少后14 day.Conclusions：这是第一次研究，以评估在六个梯度温度同时血清分析物的稳定性。每种分析物在一定温度范围内具有独特的稳定性模式。
Background: Many reports address the stability of biochemical analytes in serum. However, studies covering a wide range of storage temperatures are unavailable. Using equipment enabling precise temperature control, we investigated the effect of six different storage temperatures on serum analytes.Methods: Serum specimens from seven healthy volunteers were obtained and divided into multiple aliquots for storage at -30, -20, -10, 0, 4, and 25 degrees C. On days 1, 3, 7, 14, 28 and 56, the aliquots stored at each temperature were relocated to a deep freezer maintained at -80 degrees C. On day 60, all aliquots were measured collectively for 13 major chemistry analytes.Results: (1) At 25 degrees C, alanine aminotransferase (ALT), creatine kinase (CK), aspartate aminotransferase (AST) and total bilirubin (TBil) were very unstable especially on day 7 and later. (2) At =-20 degrees C. (5) At -30 degrees C, test results of all analytes were generally very stable except for ALT and CK, which showed noticeable reductions in activity after 14 days.Conclusions: This is the first study to assess the stability of serum analytes at six graded temperatures simultaneously. Each analyte has a unique stability pattern for a range of temperatures.