Genotoxicity, recombinogenicity and cellular preneoplasic transformation induced by Vitamin A supplementation
Genotoxicity, recombinogenicity and cellular preneoplasic transformation induced by Vitamin A supplementation
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DOI：
10.1016/s1383-5718(03)00155-4
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发表时间：
2003-08-05
影响因子：
1.9
通讯作者：
Moreira, JCF
中科院分区：
文献类型：
--
作者：
Klamt, F;Dal-Pizzol, F;Moreira, JCF
尽管是最早被发现的维生素之一，但维生素A的全部生物活性仍然不完整。越来越多的证据表明，饮食中的视黄醇明显增强了致癌作用。由于DNA损伤是一个公认的致癌诱导剂，本研究的目的是测试可能的遗传毒性作用的饮食视黄醇，使用不同类型的生物测定。通过SMART测试测量，视黄醇引起黑腹果蝇幼虫重组活性增加。在哺乳动物细胞培养物中，通过彗星试验测定，在终末分化的大鼠支持细胞中，视黄醇降解诱导的DNA双链断裂（DSB）和单链断裂（SSB）、细胞周期进展和增殖灶形成，以及中国仓鼠肺成纤维细胞（V79细胞）中DNA断裂增加。总之，我们的研究结果表明，视黄醇导致DNA损伤和染色体重排，这可能会干扰关键的生理过程，并导致细胞周期的进展和终末分化的哺乳动物细胞的肿瘤前转化。(C)2003 Elsevier B.V.保留所有权利。
In spite of being one of the first vitamins to be discovered, the full range of biological activities of Vitamin A remains incomplete. A growing body of evidence has demonstrated an apparent enhancement of carcinogenesis, induced by dietary retinol. Since DNA damage is a well-recognized inducer of carcinogenesis, the aim of this study was to test the possible genotoxic effect of dietary retinol, using different types of bioassays. Retinol caused an increased recombinogenic activity in Drosophila melanogaster larvae as measured by the SMART test. In mammalian cell cultures, retinol supplementation-induced DNA double-strands breaks (DSB) and single-strands breaks (SSB), cell cycle progression and proliferative focus formation in terminal-differentiated rat Sertoli cells and increased DNA fragmentation in Chinese hamster lung fibroblasts (V79 cells), as measured by the comet assay. Altogether, our results suggest that retinol causes DNA damage and chromosomal rearrangements, which may disturbs key physiological processes and lead to cell cycle progression and preneoplasic transformation of terminal-differentiated mammalian cells. (C) 2003 Elsevier B.V. All rights reserved.