Estimation of genetic variation at the DNA level from restriction endonuclease data.
Estimation of genetic variation at the DNA level from restriction endonuclease data.
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根据限制性内切酶数据估计 DNA 水平的遗传变异。
DOI：
10.1073/pnas.78.6.3748
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发表时间：
1981
影响因子：
11.1
通讯作者：
Harris,H
中科院分区：
文献类型：
--
作者：
Ewens,WJ;Spielman,RS;Harris,H
当数据是通过使用限制性内切酶获得时，我们考虑对自然种群中的遗传变异的估计。在限制性内切酶技术中，只要出现适合内切酶的识别序列，就会考虑并剪切特定的DNA片段。我们考虑用一个或一组限制性内切酶以这种方式处理随机的同源DNA片段样本时产生的数据。产生的片段的数量和大小指示识别序列的位置和频率(或者，对于一组限制性内切酶，每个识别序列的位置和频率)。样本中的这些频率构成了估计种群遗传变异量的基础。
We consider the estimation of the genetic variation in a natural population when the data are obtained by the use of restriction endonucleases. Under the restriction endonuclease technique, a particular DNA segment is considered and cut wherever a recognition sequence appropriate to the endonuclease occurs. We consider data generated when a random sample of homologous DNA segments is treated in this way with one or a battery of restriction endonucleases. The numbers and sizes of the fragments that result indicate the locations and the frequencies of the recognition sequence (or, with a battery of restriction endonucleases, of each recognition sequence). These frequencies in the sample form the basis for an estimate of the amount of genetic variation in the population.