Expression of ADAMs ("a disintegrin and metalloprotease") in the human lung
Expression of ADAMs ("a disintegrin and metalloprotease") in the human lung
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DOI：
10.1007/s00428-009-0748-4
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发表时间：
2009-04-01
期刊：
影响因子：
3.5
通讯作者：
Timens, Wim
中科院分区：
文献类型：
--
作者：
Dijkstra, Antoon;Postma, Dirkje S.;Timens, Wim
鉴于“去整合素和金属蛋白酶”（ADAM）与呼吸系统疾病的关联，我们评估了各种亚当斯在人肺组织中的表达。肺组织来自9名接受肺癌手术或肺气肿肺移植的个体。此外，使用16 HBE 14 o-（人支气管上皮）和A549（肺泡II型上皮样）细胞系。使用识别不同ADAM结构域的抗体进行免疫组织化学。亚当斯主要分布于支气管上皮。ADAM 8和ADAM 10在上皮各层均呈弥漫性表达。ADAM 9、ADAM 17和ADAM 19主要在上皮的顶端部分表达，ADAM 33主要在基底上皮细胞中强烈表达。在平滑肌中，ADAM 19和ADAM 17强表达，ADAM 33也是如此，尽管这种表达较弱。ADAM 33在血管内皮细胞强表达。所有的亚当斯通常在炎症细胞中表达。亚当斯在肺中的典型分布，特别是在上皮中，是有趣的，并表明局部功能。由于大多数亚当斯参与（促）炎症介质和生长因子的释放，因此它们可能在第一道防线和气道修复事件的启动中发挥重要作用。
In view of the associations of "a disintegrin and metalloprotease" (ADAM) with respiratory diseases, we assessed the expression of various ADAMs in human lung tissue. Lung tissue was obtained from nine individuals who underwent surgery for lung cancer or underwent lung transplantation for emphysema. Also, 16HBE 14o- (human bronchial epithelial) and A549 (alveolar type II epithelium-like) cell lines were used. Immunohistochemistry was performed with antibodies recognizing different ADAM domains. The ADAMs were typically distributed over the bronchial epithelium. ADAM8 and ADAM10 were expressed diffusely in all layers of the epithelium. ADAM9, ADAM17, and ADAM19 were predominantly expressed in the apical part of the epithelium, and ADAM33 was predominantly and strongly expressed in basal epithelial cells. In smooth muscle, ADAM19 and ADAM17 were strongly expressed, as was ADAM33, though this expression was weaker. ADAM33 was strongly expressed in vascular endothelium. All ADAMs were generally expressed in inflammatory cells. The typical distribution of ADAMs in the lung, especially in the epithelium, is interesting and suggests a localized function. As most ADAMs are involved in release of (pro-) inflammatory mediators and growth factors, they may play an important role in the first line of defense and in initiation of repair events in the airways.