Genome-wide binding analysis reveals that ANAC060 directly represses sugar-induced transcription of ABI5 in Arabidopsis
Genome-wide binding analysis reveals that ANAC060 directly represses sugar-induced transcription of ABI5 in Arabidopsis
复制标题
全基因组结合分析表明 ANAC060 直接抑制拟南芥中糖诱导的 ABI5 转录
DOI：
10.1111/tpj.14777
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Teng S
中科院分区：
文献类型：
--
作者：
Yu B;Wang Y;Zhou H;Li P;Liu C;Chen S;Peng Y;Zhang Y;Teng S
植物的糖分状况是影响生长和发育的信号。高水平的糖抑制幼苗建立的现象已被广泛用于深入了解糖信号通路。在ANAC 060位点已鉴定出天然等位基因变异。拟南芥哥伦比亚生态型产生一个短的ANAC 060蛋白，没有一个组成性位于细胞核的跨膜结构域，导致糖不敏感性时，过表达。在这项研究中，我们使用在ananac 060背景下表达功能性ANAC 060-GFP融合蛋白的转基因系，通过染色质免疫沉淀测序生成了ANAC 060的全基因组DNA结合图谱。共鉴定了3282个与ANAC 060结合位点相关的基因。这些基因在生物和非生物胁迫反应中富集，并且G-box结合基序在ANAC 060结合的基因组区域中高度富集。表达微阵列分析鉴定了8350个基因，这些基因的活性在anac 060突变体和糖处理后发生了改变。聚类分析表明，ANAC 060减弱糖调节基因的表达。ANAC 060的直接靶基因包括同等数量的被ANAC 060上调或下调的基因。这些靶基因的各种功能表明ANAC 060具有多种功能。我们的研究结果表明，ANAC 060直接结合到ABI 5的启动子，并抑制糖诱导的ABI 5的转录。遗传数据表明ABI 5在糖和脱落酸反应中对ANAC 060具有上位性。
The sugar status of a plant acts as a signal affecting growth and development. The phenomenon by which high levels of sugars inhibit seedling establishment has been widely used to gain insight into sugar‐signaling pathways. Natural allelic variation has been identified at theANAC060locus. The Arabidopsis Columbia ecotype produces a short ANAC060 protein without a transmembrane domain that is constitutively located to the nucleus, causing sugar insensitivity when overexpressed. In this study, we generated a genome‐wide DNA‐binding map of ANAC060 via chromatin immunoprecipitation sequencing using transgenic lines that express a functional ANAC060–GFP fusion protein in ananac060background. A total of 3282 genes associated with ANAC060‐binding sites were identified. These genes were enriched in biotic and abiotic stress responses, and the G‐box binding motif was highly enriched in ANAC060‐bound genomic regions. Expression microarray analysis resulted in the identification of 8350 genes whose activities were altered in theanac060mutant and upon sugar treatment. Cluster analysis revealed thatANAC060attenuates sugar‐regulated gene expression. Direct target genes of ANAC060 included equivalent numbers of genes that were upregulated or downregulated byANAC060. The various functions of these target genes indicate that ANAC060 has several functions. Our results demonstrate that ANAC060 directly binds to the promoter ofABI5and represses the sugar‐induced transcription ofABI5. Genetic data indicate thatABI5is epistatic toANAC060in both sugar and abscisic acid responses.