A reagentless signal-on architecture for electronic, aptamer-based sensors via target-induced strand displacement
A reagentless signal-on architecture for electronic, aptamer-based sensors via target-induced strand displacement
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DOI：
10.1021/ja056555h
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发表时间：
2005-12-28
影响因子：
15
通讯作者：
Heeger, AJ
中科院分区：
文献类型：
--
作者：
Xiao, Y;Piorek, BD;Heeger, AJ
凝血酶结合可稳定适体的替代 G-四链体构象，释放亚甲蓝 (MB) 标记的寡核苷酸以产生灵活的单链 DNA 元件。这使得 MB 标签与金电极表面碰撞，在凝血酶浓度低至 ∼3 nM 时产生易于检测的法拉第电流。
Thrombin binding stabilizes the alternative G-quadruplex conformation of the aptamer, liberating the methylene blue (MB)-tagged oligonucleotide to produce a flexible, single-stranded DNA element. This allows the MB tag to collide with the gold electrode surface, producing a readily detectable Faradaic current at thrombin concentrations as low as ∼3 nM.