Cellular Physiology Cellular Physiology Cellular Physiology Cellular Physiology Cellular Physiology Estimation of Action Potential Changes from Field Potential Recordings in Multicellular Mouse Cardiac Myocyte Cultures Key Words Microelectrode Array @bullet Action Potential Upstroke @bullet Action P
Cellular Physiology Cellular Physiology Cellular Physiology Cellular Physiology Cellular Physiology Estimation of Action Potential Changes from Field Potential Recordings in Multicellular Mouse Cardiac Myocyte Cultures Key Words Microelectrode Array @bullet Action Potential Upstroke @bullet Action P
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通讯作者：
Biochemistr;Biochemistr And Biochemistry;M. Halbach;U. Egert;J. Hescheler;K. Banach
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作者：
Biochemistr;Biochemistr And Biochemistry;M. Halbach;U. Egert;J. Hescheler;K. Banach
背景：使用基板集成微电极阵列（MEA）对电活动进行细胞外记录，能够对收缩的多细胞心脏制剂进行非侵入性长期监测。然而，为了不仅表征场电位 (FP) 记录中的激励传播和传导速度，还需要对 FP 进行更严格的分析。因此，在本研究中，我们的目标是通过同时记录 AP 和 FP 来表征内在动作电位 (AP) 参数。方法：使用由 60 个基板集成电极组成的 MEA 记录离体小鼠胚胎心肌细胞多细胞制剂的 FP 波形。在单个微电极和药物干预附近同时进行电流钳记录使我们能够将 FP 和 AP 成分及其时间进程关联起来。结果：实验揭示了 AP 上升时间和 FP 上升时间之间以及 AP 持续时间和 FP 持续时间之间的线性关系。此外，可以识别电压依赖性Na +-和Ca 2+-电流对FP的直接贡献。结论：FP 的表征使我们首次能够通过长期培养过程中多细胞心脏制剂中的非侵入性记录来估计 AP 变化以及各个电流成分对 AP 的贡献。
Background: Extracellular recordings of electrical activity with substrate-integrated microelectrode arrays (MEAs) enable non-invasive long-term monitoring of contracting multicellular cardiac preparations. However, to characterize not only the spread of excitation and the conduction velocity from field potential (FP) recordings, a more rigorous analysis of FPs is necessary. Therefore in this study we aim to characterize intrinsic action potential (AP) parameters by simultaneous recording of APs and FPs. Methods: A MEA consisting of 60 substrate-integrated electrodes is used to record the FP-waveform from multicellular preparations of isolated embryonic mouse cardiomyocytes. Simultaneous current clamp recordings in the vicinity of individual microelectrodes and pharmacological interventions allowed us to correlate FP and AP components and their time course. Results: The experiments revealed a linear relationship between AP rise time and FP rise time as well as a linear relationship between AP duration and FP duration. Furthermore a direct contribution of the voltage dependent Na +-and Ca 2+-current to the FP could be identified. Conclusion: The characterization of the FP allows us for the first time to estimate AP changes and the contribution of individual current components to the AP by the help of non-invasive recording within a multicellular cardiac preparation during long-term culture.