Diamond-type lipid cubic phase with large water channels
Diamond-type lipid cubic phase with large water channels
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DOI：
10.1021/ja034578v
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发表时间：
2003-06-18
影响因子：
15
通讯作者：
Campitelli, A
中科院分区：
文献类型：
--
作者：
Angelov, B;Angelova, A;Campitelli, A
本文描述了一种金刚石立方相与大的水通道，并确定了在立方单油酸甘油酯/辛基葡萄糖苷/水系统的时间分辨同步X射线衍射数据的基础上的双层厚度的温度依赖性。X射线衍射研究建立了一个钻石型脂质立方相与大的水通道（Dlarge），这是以前没有报道过的。它是一种独特的相，不同于具有正常水通道（Dnormal）的金刚石立方相。较大的通道可能允许在脂质立方相中提高生物分子的包封效率。在热扫描期间记录的X射线衍射图案显示从D大到D正常的立方-立方结构转变。所得到的立方相显示大得多的晶格间距相比，在完全水合的纯单油酸甘油酯。
This paper describes a diamond cubic phase with large water channels and determines the temperature dependence of the bilayer thickness in the cubic monoolein/octylglucoside/water system based on time-resolved synchrotron X-ray diffraction data. The X-ray diffraction study established a diamond-type lipid cubic phase with large water channels (Dlarge), which has not been previously reported. It is a distinct phase, different from the diamond cubic phase with normal water channels (Dnormal). The larger channels might allow an enhanced entrapment efficiency of biomolecules in lipid cubic phases. The X-ray diffraction patterns recorded during a thermal scan showed a cubic−cubic structural transition from Dlargeto Dnormal. The obtained cubic phases displayed much larger lattice spacings as compared to those of pure monoolein at full hydration.