Genomic diversity in Campylobacter jejuni:: Identification of C-jejuni 81-176-specific genes
Genomic diversity in Campylobacter jejuni:: Identification of C-jejuni 81-176-specific genes
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DOI：
10.1128/jcm.43.5.2330-2338.2005
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发表时间：
2005-05-01
影响因子：
9.4
通讯作者：
Stintzi, A
中科院分区：
文献类型：
--
作者：
Poly, F;Threadgill, D;Stintzi, A
空肠弯曲菌NCTC 11168全基因组序列自2000年公布以来，已有证据表明空肠弯曲菌具有高度的基因组多样性。为了研究这种多样性，利用鸟枪式基因芯片技术对一株未测序的空肠弯曲菌81-176DNA序列与空肠弯曲菌NCTC 11168进行了比较分析。获得了该病原菌特有的长达63kb的新染色体DNA序列。通过编码至少40个氨基酸的不间断编码区的存在，确定了86个开放阅读框。此外，这项研究表明，全质粒散弹枪微阵列方法是有效的，并提供了两个密切相关基因组之间不同的DNA区域的全面覆盖。该弯曲菌携带的两个质粒pTet和pVir也被测序，覆盖率分别为2.5倍和2.9倍，分别占其完整核苷酸序列的72%和92%。独特的染色体基因编码蛋白质，涉及胶囊和脂寡糖的生物合成，限制和修饰系统，以及呼吸代谢。这些独特的基因中有几个可能与空肠弯曲菌81-176的健康和毒力有关。有趣的是，将空肠弯曲菌81-176的独特基因与空肠弯曲菌ATCC 43431的基因进行比较，发现了一个可能编码TRAG样蛋白的基因。该基因产物可能与空肠弯曲菌侵袭上皮细胞的机制有关。总之，这项研究扩展了空肠弯曲菌的基因谱系，从而可以构建一个复合的、更全面的空肠弯曲菌微阵列。
Since the publication of the complete genomic sequence of Campylobacter jejuni NCTC 11168 in February 2000, evidence has been compiling that suggests C. jejuni strains exhibit high genomic diversity. In order to investigate this diversity, the unique genomic DNA sequences from a nonsequenced Campylobacter strain, C. jejuni 81-176, were identified by comparison with C. jejuni NCTC 11168 by using a shotgun DNA microarray approach. Up to 63 kb of new chromosomal DNA sequences unique to this pathogen were obtained. Eighty-six open reading frames were identified by the presence of uninterrupted coding regions encoding a minimum of 40 amino acids. In addition, this study shows that the whole-plasmid shotgun microarray approach is effective and provides a comprehensive coverage of DNA regions that differ between two closely related genomes. The two plasmids harbored by this Campylobacter strain, pTet and pVir, were also sequenced, with coverages of 2.5- and 2.9-fold, respectively, representing 72 and 92% of their complete nucleotide sequences. The unique chromosomal genes encode proteins involved in capsule and lipooligosaccharide biosynthesis, restriction and modification systems, and respiratory metabolism. Several of these unique genes are likely associated with C. jejuni 81-176 fitness and virulence. Interestingly, the comparison of C. jejuni 81-176 unique genes with those of C. jejuni ATCC 43431 revealed a single gene which encodes a probable TraG-like protein. The product of this gene might be associated with the mechanism of C. jejuni invasion into epithelial cells. In conclusion, this study extends the repertoire of C. jejuni genes and thus will permit the construction of a composite and more comprehensive microarray of C. jejuni.