THE ETS1 TRANSCRIPTION FACTOR IS WIDELY EXPRESSED DURING MURINE EMBRYO DEVELOPMENT AND IS ASSOCIATED WITH MESODERMAL CELLS INVOLVED IN MORPHOGENETIC PROCESSES SUCH AS ORGAN FORMATION
THE ETS1 TRANSCRIPTION FACTOR IS WIDELY EXPRESSED DURING MURINE EMBRYO DEVELOPMENT AND IS ASSOCIATED WITH MESODERMAL CELLS INVOLVED IN MORPHOGENETIC PROCESSES SUCH AS ORGAN FORMATION
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DOI：
10.1073/pnas.90.16.7588
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发表时间：
1993-08-15
影响因子：
11.1
通讯作者：
SETH, A
中科院分区：
文献类型：
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作者：
KOLA, I;BROOKES, S;SETH, A
Ets基因家族编码一类转录因子。Ets1主要在新生和成年小鼠的淋巴器官中表达，而Ets2在检查的每个器官中表达。在这项研究中，我们调查的Ets1和Ets2在小鼠胚胎发育过程中的表达。我们的数据表明，Ets1表达增加，在植入后的胚胎和器官发生过程中，它是在所有器官的第15天的胚胎研究。在胎儿后期，Ets1表达主要在淋巴组织、脑和经历分支形态发生的器官（例如，肺），但在其他器官如胃和肠中显著减少。在新生儿发育中，Ets1仅在淋巴器官和大脑中表达。原位杂交分析表明Ets1的表达发生在发育器官的间充质细胞、神经系统和骨形成中。此外，Ets1的表达在诱导分化为中胚层样细胞的P19细胞中上调。另一方面，Ets2在分化和未分化的P19和F9细胞中以及在所研究的胚胎、新生和成年小鼠的所有器官中表达。这些数据表明，Ets1在与器官形成和组织建模等形态发生过程相关的中胚层细胞中起着重要作用，而Ets2在细胞中起着更重要的作用。
The Ets family of genes encodes a class of transcription factors. Ets1 is predominantly expressed in the lymphoid organs of neonatal and adult mice, whereas Ets2 is expressed in every organ examined. In this study, we investigate the expression of Ets1 and Ets2 during murine embryonic development. Our data show that Ets1 expression increases in embryos after implantation and during organogenesis such that it is expressed in all the organs of day-15 embryos studied. In later fetal stages, Ets1 expression is predominant in the lymphoid tissues, brain, and organs that are undergoing branching morphogenesis (e.g., lung) but is dramatically reduced in other organs such as the stomach and intestine. In neonatal development, Ets1 is expressed only in the lymphoid organs and brain. In situ hybridization analysis demonstrates that expression of Ets1 occurs in mesenchymal cells of developing organs, in the nervous system, and in forming bone. Furthermore, expression of Ets1 is upregulated in P19 cells induced to differentiate into mesoderm-like cells. Ets2, on the other hand, is expressed in differentiated and undifferentiated P19 and F9 cells and in all organs of embryonic, neonatal, and adult mice studied. These data suggest that Ets1 plays an important role in mesodermal cells associated with morphogenetic processes such as organ formation and tissue modeling, whereas Ets2 plays a more fundamental role in cells.