Genetic hybridization of highly active exogenous functional proteins into silk-based materials using "light-clothing'' strategy
Genetic hybridization of highly active exogenous functional proteins into silk-based materials using "light-clothing'' strategy
复制标题
利用“轻衣”策略将高活性外源功能蛋白基因杂交到丝基材料中
DOI：
10.1016/j.matt.2021.03.020
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发表时间：
2021
期刊：
影响因子：
18.9
通讯作者：
Zhao Aichun
中科院分区：
文献类型：
--
作者：
Long Dingpei;Cheng Xian;Hao Zhanzhang;Sun Jianfeng;Umuhoza Diane;Liu Yanping;Chen Lili;Xiang Zhonghuai;Yang Fang;Zhao Aichun
利用转基因家蚕将感兴趣的外源功能蛋白(S)与蚕丝进行遗传杂交，为设计蚕丝基材料提供了良好的生物合成平台。为了确保杂交成功，来自天然丝素的添加结构域通常与POI融合形成融合POI(F-POI)，但它们往往导致多余的翻译后修饰，损害正常折叠，并严重削弱POI的活性。在此，提出了“轻装化”策略，即合理裁剪可加性域的冗余部分。根据绿色荧光蛋白作为POI标记的可视化，该策略避免了F-POI合成中多余的翻译后修饰，并使其正常分泌和提取。以表皮生长因子为模型功能POI，展示了F-POI与蚕丝混合物的材料制备通用性。这一策略增加了F-POI与天然POI的结构相似性，显著提高了F-POI的活性，使其达到与商业POI相同的水平，从而促进了这些先进的生物合成平台在开发高性能材料方面的应用。
Genetic hybridization of exogenous functional protein(s) of interest (POI) with silk using transgenic silkworms provides promising bio-synthesis platforms to design silk-based materials. To ensure successful hybridization, additive domains from natural silk are routinely fused with POI to form fusion POI (F-POI), but they often lead to superfluous post-translational modifications, compromise normal folding, and severely impair the activity of POI. Here, "light-clothing" strategy, i.e., rationally trimming redundant parts of additive domains, is proposed. As visualized by green fluorescent protein as marker POI, this strategy avoids supernumerary post-translational modifications in F-POI synthesis, and enables its normal secretion and extraction. Applying epidermal growth factor as model functional POI, material fabrication versatility of the extracted mixture of F-POI and silk is demonstrated. This strategy increases structural similarity between F-POI and native POI and remarkably enhances F-POI activity to the same level as commercial POI, which facilitates applications of these advanced bio-synthesis platforms for developing high-performance materials.