COMPLEMENTATION ANALYSIS AND DELETION MAPPING OF ESCHERICHIA-COLI MUTANTS DEFECTIVE IN CHEMOTAXIS
COMPLEMENTATION ANALYSIS AND DELETION MAPPING OF ESCHERICHIA-COLI MUTANTS DEFECTIVE IN CHEMOTAXIS
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DOI：
10.1128/jb.135.1.45-53.1978
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发表时间：
1978-01-01
影响因子：
3.2
通讯作者：
PARKINSON, JS
中科院分区：
文献类型：
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作者：
PARKINSON, JS
E.检测了大肠杆菌与特异性λ弗拉转导质粒的互补和重组。这些突变体的复杂的互补行为发现以前在F-总理测试可以在很大程度上占基因内互补和极性效应。cheA类的突变体定义了2个基因，cheA和cheW，它们似乎是共转录的。切布类的突变体定义了四个基因cheX、切布、cheY和cheZ，它们也构成了一个转录单位。在cheA、cheW、cheX或cheY功能上有缺陷的突变体平滑地游动，几乎没有或没有翻滚，但是切布或cheZ突变体表现出非常高的翻滚率。这些功能可能参与启动或控制鞭毛旋转的变化，以响应趋化刺激。
Motile, but generally nonchemotactic (che) mutants of E. coli were examined for complementation and recombination with specialized .lambda.fla transducing phages. The complex complementation behavior of these mutants found previously in F-prime tests could largely be accounted for by intragenic complementation and by polarity effects. Mutants of the cheA class defined 2 genes, cheA and cheW, which appeared to be co-transcribed. Mutants of the cheB class defined four genes, cheX, cheB, cheY and cheZ, which also constituted a transcriptional unit. Mutants defective in cheA, cheW, cheX or cheY function swam smmothly, with little or no tumbling, but cheB or cheZ mutants exhibited very high tumbling rates. These functions are probably involved in initiating or controlling changes in flagellar rotation in response to chemotactic stimuli.