Responses of BrdU label-retaining dental pulp cells to allogenic tooth transplantation into mouse maxilia
Responses of BrdU label-retaining dental pulp cells to allogenic tooth transplantation into mouse maxilia
复制标题
保留 BrdU 标记的牙髓细胞对小鼠上颌同种异体牙移植的反应
DOI：
10.1007/s00418-011-0868-1
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Ohshima H
中科院分区：
文献类型：
--
作者：
Mutoh N;Nakatomi M;Ida-Yonemochi H;Nakagawa E;Tani-Ishii N;Ohshima H
Recently, we demonstrated that a pulse of BrdU given to prenatal animals reveals the existence of slow-cycling long-term label-retaining cells (LRCs), putative adult stem or progenitor cells, which reside in the dental pulp. This study aims to clarify responses of LRCs to allogenic tooth transplantation into mouse maxilla using prenatal BrdU-labeling, in situ hybridization forosteopontinandperiostin,and immunohistochemistry for BrdU, nestin, and osteopontin. The upper-right first molars were allografted in the original socket between BrdU-labeled and non-labeled mice or between GFP transgenic and wild-type mice. Tooth transplantation caused degeneration of the odontoblast layer, resulting in the disappearance of nestin-positive reactions in the dental pulp. On postoperative days 5–7, tertiary dentin formation commenced next to the preexisting dentin where nestin-positive odontoblast-like cells were arranged in the successful cases. In BrdU-labeled transplanted teeth, dense LRCs were maintained in the center of the dental pulp beneath the odontoblast-like cells including LRCs, whereas LRCs disappeared in the area surrounding the bone-like tissue. In contrast, LRCs were not recognized in the pulp chamber of non-labeled transplants through the experimental period. Tooth transplantation using GFP mice demonstrated that the donor cells constituted the dental pulp of the transplant except for endothelial cells and some migrated cells, and the periodontal tissue was replaced by host-derived cells except for epithelial cell rests of Malassez. These results suggest that the maintenance of BrdU label-retaining dental pulp cells play a role in the regeneration of odontoblast-like cells in the process of pulpal healing following tooth transplantation.
