Comparative proteomic analysis of mouse embryonic stem cells and neonatal-derived cardiomyocytes
Comparative proteomic analysis of mouse embryonic stem cells and neonatal-derived cardiomyocytes
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DOI：
10.1016/j.bbrc.2006.08.151
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发表时间：
2006-10-27
影响因子：
3.1
通讯作者：
Salekdeh, Ghasem Hosseini
中科院分区：
文献类型：
--
作者：
Baharvand, Hossein;Hajheidari, Mohsen;Salekdeh, Ghasem Hosseini
多能性胚胎干细胞（ESC）通过胚胎样聚集体自发分化为心肌细胞。在这些细胞的其他潜在应用（如细胞治疗）得以实现之前，对ESCs分子条件的透彻理解是必要的。我们应用双向电泳分析和比较自发性小鼠胚胎干细胞衍生的心肌细胞（ESC-DCs），未分化的小鼠胚胎干细胞，和胚胎干细胞衍生的心肌细胞（N-DCs）的蛋白质组谱。在ESC-DCs和N-DCs上检测到的95%的蛋白质可以彼此精确配对，而只有20%的ESC蛋白质可以与ESC-DCs和N-DCs上的蛋白质可靠地匹配，这表明它们之间具有惊人的相似性。在上述三种细胞类型中鉴定了60种蛋白质后，我们试图为它们的差异表达模式提供可能的解释，并讨论它们与细胞生物学的相关性。本研究为了解分化心肌细胞的基因表达模式提供了新的见解，并进一步证明了ESC-DCs和N-DCs之间的密切关系。(c)2006爱思唯尔公司All rights reserved.
Pluripotent embryonic stem cells (ESCs) spontaneously differentiate via embryo-like aggregates into cardiomyocytes. A thorough understanding of the molecular conditions in ESCs is necessary before other potential applications of these cells such as cell therapy can be materialized. We applied two dimensional electrophoresis to analyze and compare the proteome profiling of spontaneous mouse ESC-derived cardiomyocytes (ESC-DCs), undifferentiated mouse ESCs, and neonatal-derived cardiomyocytes (N-DCs). Ninety-five percent of the proteins detected on the ESC-DCs and N-DCs could be precisely paired with one other, whereas only twenty percent of the ESC proteins could be reliably matched with those on the ESC-DCs and N-DCSs, suggesting a striking similarity between them. Having identified sixty proteins in the said three cell types, we sought to provide possible explanations for their differential expression patterns and discuss their relevance to cell biology. This study provides a new insight into the gene expression pattern of differentiated cardiomyocytes and is further evidence for a close relation between ESC-DCs and N-DCSs. (c) 2006 Elsevier Inc. All rights reserved.