Cellular expression of isoforms of endothelin-converting enzyme-1 (ECE-1c, ECE-1b and ECE-1a) and endothelin-converting enzyme-2
Cellular expression of isoforms of endothelin-converting enzyme-1 (ECE-1c, ECE-1b and ECE-1a) and endothelin-converting enzyme-2
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DOI：
10.1097/00005344-200036001-00006
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发表时间：
2000-01-01
影响因子：
3
通讯作者：
Kuc, RE
中科院分区：
文献类型：
--
作者：
Davenport, AP;Kuc, RE
我们的目的是比较内皮素转换酶-1（ECE-1）亚型和ECE-2的细胞表达，使用免疫细胞化学在正常和患病的人体组织。强烈的ECE-1b免疫反应性存在于肾和肺上皮细胞内，ECE-1c，ECE-1a和ECE-2抗血清显示较低水平的染色。用所有抗血清（ECE-1a除外）在具有一定范围的管腔直径的肾和肺血管以及软脑膜动脉和穿透脑的脑内血管的内皮内检测染色。ECE-1b，ECE-1c和ECE-2免疫反应定位于血管周围的星形胶质细胞和大脑皮层的神经元过程。在病变血管中，ECE-1c，ECE-1b和ECE-2抗血清染色巨噬细胞浸润冠状动脉内的动脉粥样硬化斑块。这些结果表明，ECE-1b和ECE-2可能广泛表达于人类正常组织中，抑制病理生理组织中细胞如巨噬细胞表达的ECE-1亚型和ECE-2可能是心血管疾病的另一个治疗靶点。
Our aim was to compare the cellular expression of endothelin-converting enzyme-1 (ECE-1) isoforms and ECE-2 using immunocytochemistry in normal and diseased human tissue. Intense ECE-1b immunoreactivity was present within renal and pulmonary epithelial cells with lower levels of staining displayed by ECE-1c, ECE-1a and ECE-2 antisera. Staining was detected with all antisera (except ECE-1a) within the endothelium of renal and pulmonary vessels having a range of lumen diameters as well as pial arteries and intracerebral vessels penetrating brain. ECE-1b, ECE-1c and ECE-2 immunoreactivity was localized to perivascular astrocytes and neuronal processes in the cerebral cortex. In diseased vessels, ECE-1c, ECE-1b and ECE-2 antisera stained macrophages infiltrating atherosclerotic plaques within coronary arteries. These results suggest ECE-1b and ECE-2 may be widely expressed in normal tissue from humans and inhibition of ECE-1 isoforms and ECE-2 expressed by cells such as macrophages in pathophysiological tissue may be an additional therapeutic target in cardiovascular disease.