Synthesis and characterization of size-selective nanoporous polymeric adsorbents for blood purification
Synthesis and characterization of size-selective nanoporous polymeric adsorbents for blood purification
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DOI：
10.1016/j.seppur.2009.01.016
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发表时间：
2009-05-07
影响因子：
8.6
通讯作者：
Hoenich, N. A.
中科院分区：
文献类型：
--
作者：
Malik, D. J.;Webb, C.;Hoenich, N. A.
用膜乳化法合成了中位直径在40 ~ 300 μ m之间的交联聚苯乙烯-二乙烯基苯吸附剂微球，并在4-10 nm范围内增加了介孔比例。目的是开发一种吸附剂，用于选择性去除中分子量尿毒症毒素（尺寸范围0.5-20 kDa），例如β（2）-微球蛋白（11.8 kDa），同时排除尺寸较大的血液蛋白，例如血清白蛋白（MW 69 kDa）。使用反相尺寸排阻色谱法，使用鸡蛋溶菌酶（β（2）-微球蛋白的替代品）和人血清白蛋白作为分批吸附研究的模型蛋白，证明了尺寸排阻的作用。吸附剂样品具有纳米多孔结构，孔主要小于10 nm，成功地尺寸排除血清白蛋白，同时显示出显着的吸附能力的中分子量溶菌酶（14.3 kDa）。(C)2009 Elsevier B. V.保留所有权利。
Crosslinked polystyrene co-divinylbenzene adsorbent microspheres with median diameters between 40 and 300 mu m, with an enhanced proportion of mesopores in the range 4-10 nm, have been synthesized using a membrane emulsification technique. The aim was to develop an adsorbent for the selective removal of middle molecular weight uraemic toxins (size range 0.5-20 kDa), e.g. beta(2)-microglobulin (11.8 kDa), whilst size-excluding larger blood proteins, e.g. serum albumin (MW 69 kDa). Using inverse size exclusion chromatography, the role of size exclusion has been demonstrated using hen egg lysozyme (a surrogate for beta(2)-microglobulin) and human serum albumin as model proteins for batch adsorption studies. The adsorbent sample possessing a nanoporous structure with pores predominantly smaller than 10 nm successfully size-excludes serum albumin whilst displaying significant adsorption capacity for the middle molecular weight lysozyme (14.3 kDa). (C) 2009 Elsevier B.V. All rights reserved.