Stimulation of DNA synthesis in rabbit bladder wall after partial outlet obstruction and acute overdistension.
Stimulation of DNA synthesis in rabbit bladder wall after partial outlet obstruction and acute overdistension.
复制标题
部分出口梗阻和急性过度扩张后兔膀胱壁 DNA 合成的刺激。
DOI：
10.1002/nau.1930130108
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发表时间：
1994
影响因子：
2
通讯作者：
Levin,RM
中科院分区：
文献类型：
--
作者：
Monson,FC;Wein,AJ;Eika,B;Murphy,M;Levin,RM
兔尿道膀胱连接部（UVJ）的部分出口梗阻已被用于诱导在人类中观察到的梗阻性损伤的膀胱病理学特征。本文报告的实验目的是比较雄性新西兰白色（NZW）兔膀胱部分出口梗阻或过度扩张时膀胱中DNA的3 H-胸苷（3 H-TdR）标记。共使用了18只动物。两个正常对照和12个部分阻塞动物（在第1天[D]、3D、5D、7 D、14 D和2 ID）注射（i. v.）以0.5 μ Ci/g体重的剂量使用3 H-TdR。另外4只过度扩张至最大膀胱内压的120%，立即通过导管排空，24小时后注射3 H-TdR（ID）。所有动物在注射标记物后3.5小时内处死。DNA相关放射性在阻塞后3D达到峰值，并在5D时大幅降低，尽管掺入水平仍远高于2 ID的对照水平。膀胱过度扩张后的3 H-TdR掺入ID水平约为部分梗阻后ID水平的一半。组织切片放射自显影显示组织中3 H-TdR标记的DNA分布。梗阻后1天，3 H-Tdr掺入位于尿路上皮。ID后尿路上皮的标记减少，但保持高于对照水平，直到2 ID。仅在对照组和3D膀胱中观察到标记的平滑肌细胞核，并且以相似的频率测量。内在结缔组织（ICT）（粘膜、粘膜下和壁）和外在结缔组织（ECT）（浆膜）的标记在梗阻后3D达到峰值，此后下降，但未达到对照水平。当然，ECT的标签仅限于存在ECT的膀胱（即，3 - 2ID）。虽然放射自显影图中标记细胞的分布在阻塞后ID比过度扩张后ID更易变，但通过DNA合成测量的对过度扩张的一般细胞和生化反应与部分出口阻塞后观察到的相似。由于梗阻的第一个后遗症是急性扩张，这些数据支持膀胱最初过度扩张引发细胞对梗阻的反应的说法。© 1994 Wiley‐利斯公司
Partial outlet obstruction of the rabbit urethrovesical junction (UVJ) has been used to induce pathology in the urinary bladder characteristic of obstructive damage observed in humans. The purpose of the experiments reported here was to compare the3H‐thymidine (3H‐TdR) labelling of DNA in urinary bladders of male New Zealand White (NZW) rabbits subjected to partial outlet obstruction or overdistension. A total of 18 animals was used. Two normal controls, and 12 partially obstructed animals (at I day [D], 3D, 5D, 7D, 14D, and 2ID) were injected (i.v.) with3H‐TdR at a dose of 0.5 μ‐Ci/g body weight. An additional 4 were overdistended to volumes 120% of maximum intravesical pressure, immediately emptied via the catheter, and injected with3H‐TdR 24 hr (ID) later. All animals were sacrificed up to 3.5 hr after injection of the label.DNA‐associated radioactivity reached a peak at 3D after obstruction and was reduced substantially by 5D, although the level of incorporation remained well above control levels out to 2ID. Levels of3H‐TdR incorporation ID after overdistension bladders were about half of that found ID following partial obstruction.The distribution of3H‐TdR labelled DNA in tissues was demonstrated by radioautography of histologic sections. One day following obstruction,3H‐Tdr incorporation was localized in the urothelium. Labelling of urothelium subsequent to ID was reduced but remained above control levels until 2ID. Labelled smooth muscle nuclei were observed only in control and 3D bladders, and they were measured at similar frequencies. Labelling of both intrinsic connective tissue (ICT) (mucosal, submucosal, and mural) and extrinsic connective tissue (ECT) (serosal) peaked at 3D after obstruction and declined thereafter but not to control levels. Labelling of ECT was, of course, limited to those bladders in which ECT was present (i.e., 3‐2ID). While the distribution of labelled cells in radioautograms was more variable ID after obstruction than ID after overdistension, the general cellular and biochemical responses to overdistension, as measured by DNA synthesis, are similar to those observed after partial outlet obstruction. Since the first sequela of obstruction is acute distension, these data support the assertion that the initial overdistension of the bladder initiates the cellular response to obstruction. © 1994 Wiley‐Liss, Inc.