Characterization of the RNA Transcription Profile of Bombyx mori Bidensovirus
Characterization of the RNA Transcription Profile of Bombyx mori Bidensovirus
复制标题
家蚕病毒 RNA 转录谱的表征
作者：
Li Rui;Chang Pengfei;Lu Peng;Hu Zhaoyang;Chen Keping;Yao Qin;Yu Qian
家蚕双链病毒（BmBDV）是来自双链病毒科（Bidnaviridae）的家蚕双链病毒属的单链DNA（ssDNA）病毒，其迄今仅感染昆虫。它有一个独特的基因组，含有二分DNA分子（VD 1和VD 2）。在这项研究中，我们探索了详细的转录映射完整的BmBDV基因组（VD 1和VD 2）的cDNA末端的快速扩增（RACE），逆转录定量实时PCR（RT-qPCR），荧光素酶测定。首次报道了VD 2的转录图谱。我们对转录起始位点的作图揭示了VD 1中的NS基因具有来自重叠启动子P5和P5.5的单独转录物。因此，我们的研究提供了一种策略，替代启动子的使用在BmBDV基因的表达。
Bombyx mori bidensovirus (BmBDV) is a single-stranded DNA (ssDNA) virus from the genus Bidensovirus of the Bidnaviridae family, which, thus far, solely infects insects. It has a unique genome that contains bipartite DNA molecules (VD1 and VD2). In this study, we explored the detailed transcription mapping of the complete BmBDV genome (VD1 and VD2) by rapid amplification of cDNA ends (RACE), reverse transcription quantitative real-time PCR (RT-qPCR), and luciferase assays. For the first time, we report the transcription map of VD2. Our mapping of the transcriptional start sites reveals that the NS genes in VD1 have separate transcripts that are derived from overlapping promoters, P5 and P5.5. Thus, our study provides a strategy for alternative promoter usage in the expression of BmBDV genes.