Identification of synthetic peptides derived from laminin alpha1 and alpha2 chains with cell type specificity for neurite outgrowth.
Identification of synthetic peptides derived from laminin alpha1 and alpha2 chains with cell type specificity for neurite outgrowth.
复制标题
鉴定源自层粘连蛋白 α1 和 α2 链的合成肽，对神经突生长具有细胞类型特异性。
DOI：
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发表时间：
1996
影响因子：
3.7
通讯作者：
H. Kleinman
中科院分区：
文献类型：
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作者：
B. L. Richard;M. Nomizu;Y. Yamada;H. Kleinman
层粘连蛋白-1是神经突起生长的有效促进剂。在这里，我们发现层粘连蛋白-1 α链含有肽序列，刺激附着和神经突生长在一些，但不是所有的神经元细胞。用三种层粘连蛋白-1-反应性神经源性细胞系测试一系列重叠肽的生物活性，结果分别从α 1和α 2（裂球蛋白）链球状区的相应区域鉴定出肽序列RKRLQVQLSIRT和KNRLTIELEVRT，它们对所有三种细胞系的附着和对两种细胞系的神经突生长都有活性。这些数据代表了从层粘连蛋白的a1和a2链中鉴定出对某些神经元细胞具有活性的新肽序列。肽显示出不同的附着和神经突生长的活动，这取决于细胞类型，表明细胞的响应中的细胞类型特异性。
Laminin-1 is a potent promoter of neurite outgrowth. Here we find that the laminin-1 alpha chain contains peptide sequences that stimulate attachment and neurite outgrowth in some but not all neuronal cells. Testing of a series of overlapping peptides for biological activity with three laminin-1-responsive, neuronally derived cell lines resulted in the identification of peptide sequences, RKRLQVQLSIRT and KNRLTIELEVRT, from corresponding regions of the alpha1 and alpha2 (merosin) chain globular regions, respectively, that were active for attachment for all three cell lines and for neurite outgrowth with two of the cell lines. These data represent the identification of novel peptide sequences active with certain neuronal cells from the a1 and a2 chains of laminin. The peptides showed different attachment and neurite outgrowth activities depending on the cell type, suggesting cell type specificity in the response of the cells.