FISSION YEAST CDC21+ BELONGS TO A FAMILY OF PROTEINS INVOLVED IN AN EARLY STEP OF CHROMOSOME-REPLICATION
FISSION YEAST CDC21+ BELONGS TO A FAMILY OF PROTEINS INVOLVED IN AN EARLY STEP OF CHROMOSOME-REPLICATION
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DOI：
10.1093/nar/20.21.5571
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发表时间：
1992-11-11
影响因子：
14.9
通讯作者：
KEARSEY, SE
中科院分区：
文献类型：
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作者：
COXON, A;MAUNDRELL, K;KEARSEY, SE
粟酒裂殖酵母的cdc21+基因最初是在筛选影响S期和核分裂的cdc突变体中鉴定的。在这里，我们表明，cdc21+基因产物属于一个家庭的蛋白质参与DNA复制。这些包括酿酒酵母MCM2和MCM3蛋白，它们是某些复制起点的有效功能所必需的，以及酿酒酵母CDC 46，它是染色体复制起始所必需的。cdc21突变体在一些质粒的有丝分裂维持中有缺陷，如mcm 2和mcm 3。该突变体以含有两个基因组等同物的DNA的单个细胞核停止，并保持细胞质微管构型。突变体中大多数（但不是全部）复制起点的激活可能导致一小部分基因组无法复制，这可以解释停滞表型。利用聚合酶链反应技术，我们已经确定了新的cdc21+相关基因的酿酒酵母，粟酒裂殖酵母和非洲爪蟾。我们的研究结果表明，cdc21+相关家族的个别成员在进化中是高度保守的。
The cdc21+ gene of Schizosaccharomyces pombe was originally identified in a screen for cdc mutants affecting S phase and nuclear division. Here we show that the cdc21+ gene product belongs to a family of proteins implicated in DNA replication. These include the Saccharomyces cerevisiae MCM2 and MCM3 proteins, which are needed for the efficient function of certain replication origins, and S.cerevisiae CDC46, which is required for the initiation of chromosome replication. The cdc21 mutant is defective in the mitotic maintenance of some plasmids, like mcm2 and mcm3. The mutant arrests with a single nucleus containing two genome equivalents of DNA, and maintains a cytoplasmic microtubular configuration. Activation of most, but not all, replication origins in the mutant may result in failure to replicate a small proportion of the genome, and this could explain the arrest phenotypes. Using the polymerase chain reaction technique, we have identified new cdc21+-related genes in S.cerevisiae, S.pombe and Xenopus laevis. Our results suggest that individual members of the cdc21+-related family are highly conserved in evolution.