Transcriptional regulation of Mesp1 and Mesp2 genes:: differential usage of enhancers during development
Transcriptional regulation of Mesp1 and Mesp2 genes:: differential usage of enhancers during development
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DOI：
10.1016/s0925-4773(01)00478-6
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发表时间：
2001-10-01
影响因子：
2.6
通讯作者：
Saga, Y
中科院分区：
文献类型：
--
作者：
Haraguchi, S;Kitajima, S;Saga, Y
Mesp1和Mesp2分别编码bHLH型转录因子Mesp1和Mesp2。这两个基因的表达中观察到的新生中胚层，并随后在喙preomitic中胚层。为了确定基因表达的调控机制，我们试图通过瞬时转基因分析来鉴定增强子元件。鉴定了至少两个增强子和一个抑制子，所述增强子负责所述两个基因在早期中胚层（早期中胚层增强子，EME）和前体中胚层（PSM增强子，PSME）中的表达，所述抑制子负责前体中胚层中的吻侧限制性表达。这些增强子元件的缺失研究表明，任何一个基因都可以使用相同的增强子用于早期中胚层发育，而这两个基因都可以利用单独的增强子来调节它们在体前中胚层中的表达。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
Mesp1 and Mesp2 encode bHLH-type transcription factors, Mesp1 and Mesp2, respectively. The expression of both genes is observed in the nascent mesoderm, and subsequently in the rostral presomitic mesoderm. To determine the regulatory mechanism for gene expression, we attempted to identify enhancer elements by transient transgenic analysis. At least two enhancers, which are responsible for the expression of the two genes in the early mesoderm (early mesodermal enhancer, EME) and the presomitic mesoderm (PSM enhancer, PSME), and one suppressor, which is responsible for the rostrally restricted expression in the presomitic mesoderm, were identified. Deletion studies of these enhancer elements indicate that either gene may use the same enhancer for early mesoderm development, whereas both genes may utilize separate enhancers to regulate their expression in the presomitic mesoderm. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.