B1-B cells are the main antigen presenting cells in CpG-ODN-stimulated peritoneal exudate cells
B1-B cells are the main antigen presenting cells in CpG-ODN-stimulated peritoneal exudate cells
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DOI：
10.1111/j.1348-0421.2005.tb03633.x
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发表时间：
2005-01-01
影响因子：
2.6
通讯作者：
Ogasawara, K
中科院分区：
文献类型：
--
作者：
Bamba, H;Ishigaki, H;Ogasawara, K
腹膜渗出细胞（PEC）一直被认为主要含有巨噬细胞，因此被用作抗原提呈细胞（APC）。然而，目前尚不清楚腹膜渗出液中哪些细胞具有APC功能。在这里，我们关注巨噬细胞；和B1-B细胞，检测其APC功能和细胞因子的产生。PEC纯化的B1-B细胞经cpg刺激后具有APC功能，主要产生IL-10。相比之下，尽管CpG刺激后产生IL-12并表达共刺激分子，但从PEC纯化的巨噬细胞不能将合并抗原呈递到T细胞。这些结果表明，以前关于PEC中细胞混合物功能的观点需要重新评估。综上所述，PEC的抗原提呈功能主要归因于B1-B细胞，免疫增强细胞因子的产生主要来源于腹膜巨噬细胞。
Peritoneal exudate cells (PEC) have long been used as antigen presenting cells (APC) because they have been considered to contain mainly macrophages. However, it is still unclear specifically which cells of the peritoneal exudate function as APC. Herein, we focused on macrophages; and B1-B cells of the PEC and examined their APC function and cytokine production. B1-B cells purified from PEC functioned effectively as APC after CpG-stimulation and mainly produced IL-10. In contrast, macrophages purified from PEC were not able to present incorporated antigens to T cells, despite the production of IL-12 and expression of co-stimulatory molecules after CpG stimulation. These results suggest that previously held ideas regarding the functions of the mixture of cells in the PEC need to re-evaluated. In summary the antigen presenting function of PEC was mainly attributed to B1-B cells and immunoenhancing cytokine production was dominantly derived from peritoneal macrophages.