Differences in ethidium bromide and 4′‐6‐diamidino‐2‐phenylindole staining profiles with regard to DNA fragmentation during apoptosis
Differences in ethidium bromide and 4′‐6‐diamidino‐2‐phenylindole staining profiles with regard to DNA fragmentation during apoptosis
复制标题
溴化乙锭和 4-6-二脒基-2-苯基吲哚染色图谱在细胞凋亡过程中 DNA 碎片方面的差异
DOI：
10.1080/15216549800204602
复制
发表时间：
1998
期刊：
影响因子：
4.6
通讯作者：
S. Ito
中科院分区：
文献类型：
--
作者：
M. Shono;I. Shimizu;T. Omoya;A. Hiasa;H. Honda;Yumiko Tomita;S. Ito
为了简单、直接地评价抗Fas抗体诱导小鼠肝细胞凋亡过程中的DNA片段，我们用光学荧光显微镜检测了溴化乙啶和4′- 6‐二氨基- 2‐苯并多酚染色的细胞核的荧光强度。与非凋亡细胞相比，前者对凋亡细胞核DNA的染色强度明显降低，而后者在凋亡组和非凋亡组之间的荧光强度没有差异。因此，使用光学荧光显微镜，结合这两种染色，构成了一个有用的工具，评估凋亡的DNA片段。
To simply and directly evaluate DNA fragmentation during apoptosis induced in mouse cultured hepatocytes by an anti‐Fas antibody, we examined the fluorescence intensity in cell nuclei stained with ethidium bromide and 4′‐6‐diamidino‐2‐phenyhndole by optiphoto fluorescence microscopy. The intensity of the former staining for the nuclear DNA of apoptotic cells was clearly decreased compared to that of non‐apoptotic cells, whereas no difference in the fluorescence intensity for the latter stain between the apoptotic and non‐apoptotic groups was observed. Thus, the use of optiphoto fluorescence microscopy, in conjunction with both stains, constitutes a useful tool for the evaluation of apoptotic DNA fragmentation.