Overlapping functions of different dynamin isoforms in clathrin-dependent and -independent endocytosis in pancreatic β cells
Overlapping functions of different dynamin isoforms in clathrin-dependent and -independent endocytosis in pancreatic β cells
复制标题
胰腺 β 细胞中网格蛋白依赖性和独立性内吞作用中不同动力亚型的重叠功能
DOI：
10.1016/j.bbrc.2008.04.077
复制
发表时间：
2008-06-27
影响因子：
3.1
通讯作者：
Chen, Liangyi
中科院分区：
文献类型：
--
作者：
Lu, Jingze;He, Zixuan;Chen, Liangyi
此前，我们在胰腺β细胞中发现了网格蛋白依赖性慢内吞作用和不依赖网格蛋白的快速内吞作用，两者都是由细胞质Ca2+浓度升高触发的。在当前的研究中，我们试图探索不同动力亚型在这些内吞过程中的作用。我们首先使用定量 RT-PCR 和蛋白质印迹实验证实了 INS-1 细胞中神经元特异性动力 1 和普遍存在的动力 2 的存在。通过特异性降低 INS-1 细胞中任一动力亚型的内源水平，我们发现动力 1 和动力 2 同时参与胰腺 β 细胞中网格蛋白独立和依赖的膜修复。在同时敲除动力蛋白 1 和动力蛋白 2 的细胞中，转铁蛋白内化也受到抑制。基于这些结果，我们认为不同的动力蛋白异构体在不同类型的内吞作用中发挥重叠的作用。 (C) 2008 Elsevier Inc. 保留所有权利。
Previously, we identified a clathrin-dependent slow endocytosis and a clathrin-independent fast endocytosis in pancreatic beta cells, both triggered by elevated cytoplasmic Ca2+ concentration. In the current study, we attempted to explore the roles of different dynamin isoforms in these endocytotic processes. We first confirmed the existence of both neuron-specific dynamin 1 and ubiquitous dynamin 2 in INS-1 cells using both quantitative RT-PCR and Western blot experiments. By specifically knocking down the endogenous level of either dynamin isoform from INS-1 cells, we showed that dynamin 1 and dynamin 2 simultaneously participate in the clathrin-independent and -dependent membrane retrieval in pancreatic beta cells. Transferrin internalization was also inhibited in cells with knock down of both dynamin 1 and dynamin 2. Based on these results, we argue that different dynamin isoforms play overlapping roles in different types of endocytosis. (C) 2008 Elsevier Inc. All rights reserved.