The effect of monoclonal antibody and route of immunization on the humoral immune response against Porphyromonas gingivalis
The effect of monoclonal antibody and route of immunization on the humoral immune response against Porphyromonas gingivalis
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DOI：
10.1034/j.1399-302x.2001.016003153.x
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发表时间：
2001-06-01
影响因子：
--
通讯作者：
Brady, LJ
中科院分区：
文献类型：
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作者：
van Tilburg, MLJA;Kozarov, EV;Brady, LJ
外源性抗体介导的免疫调节已被提出作为一种疫苗策略，以提高对病原微生物的免疫保护，并建议有助于被动免疫后的保护。为了测试针对牙周病原体牙龈卟啉单胞菌的粘附表位的单克隆抗体是否可以影响粘膜免疫后的体液免疫应答，用单独的牙龈卟啉单胞菌或用单克隆抗体61 BG 1.3包被的牙龈卟啉单胞菌口服或鼻内免疫BALB/c小鼠。抗-P.，牙龈卟啉单胞菌血清免疫球蛋白G（IgG）的差异在接受单克隆抗体包被的牙龈卟啉单胞菌的小鼠组与通过任一免疫途径单独接受牙龈卟啉单胞菌的小鼠组之间得到证实。免疫前单克隆抗体61 BG 1.3与牙龈卟啉单胞菌的结合不影响血清IgG亚类的分布。然而，在粘膜免疫的各种途径之间观察到亚类分布的微小差异。这些结果支持这样的假设，即特异性单克隆抗体结合到细菌疫苗可以改变体液免疫应答的质量，该生物体。
Immunomodulation mediated by exogenous antibodies has been proposed as a vaccine strategy to improve immune protection against pathogenic microorganisms and suggested to contribute to protection following passive immunization. To test whether a monoclonal antibody directed against an adhesion epitope of the periodontal pathogen Porphyromonas gingivalis could influence the humoral immune response following mucosal immunization, BALB/c mice were immunized orally or intranasally with P, gingivalis alone or P. gingivalis coated with monoclonal antibody 61BG1.3. Differences in antigenic specificity of anti-P., gingivalis serum immunoglobulin G (IgG) were demonstrated between groups of mice that received monoclonal antibody-coated P. gingivalis versus those that received P. gingivalis alone by either route of immunization. Binding of monoclonal antibody 61BG1.3 to P. gingivalis prior to immunization did not influence the serum IgG subclass distribution. However, minor differences in subclass distribution were observed between the various routes of mucosal immunization. These results support the hypothesis that specific monoclonal antibody bound to a bacterial vaccine can alter the quality of the humoral immune response to that organism.