A fluorescent and chemiluminescent difunctional mesoporous silica nanoparticle as a label for the ultrasensitive detection of cancer cells.
A fluorescent and chemiluminescent difunctional mesoporous silica nanoparticle as a label for the ultrasensitive detection of cancer cells.
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DOI：
10.1016/j.aca.2012.11.046
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发表时间：
2013-01
影响因子：
6.2
通讯作者：
Liang Tao;Chao-jun Song;Yuanjie Sun;Xiaohua Li;Yunyun Li;Boquan Jin;Z. Zhang;Kun Yang
中科院分区：
文献类型：
--
作者：
Liang Tao;Chao-jun Song;Yuanjie Sun;Xiaohua Li;Yunyun Li;Boquan Jin;Z. Zhang;Kun Yang
报道了一种新型超亮荧光和化学发光双功能介孔二氧化硅纳米颗粒(FCMSN)。一种发光染料，罗丹明6G或三(2，2‘-联吡啶)二氯六水合钚(Rubpy)，被掺杂在二氧化硅基质的纳米通道内。二氧化硅基质中的疏水基团避免了染料从开放通道中泄漏。FCMSN表面的胺基团改善了纳米粒子的改性性能。由于纳米通道被二氧化硅的网络骨架隔离，有效地减弱了固定在纳米通道中的荧光染料的内过滤效应所产生的荧光猝灭。得到的90 nm二氧化硅粒子的量子产率约为61%。与荧光核壳纳米粒子相比，化学发光试剂可以自由进入纳米粒子与荧光染料反应产生化学发光。结果表明，FCMSN既是荧光标记，又是化学发光标记。在生物应用中，NaIO4氧化法被证明优于戊二醛氧化法。氨基酸量可以影响FCMSN的特异性。荧光显微镜成像表明，FCMSN具有良好的生物应用前景。
A new kind of ultrabright fluorescent and chemiluminescent difunctional mesoporous silica nanoparticle (FCMSN) is reported. A luminescent dye, Rhodamine 6G or tris(2,2′-bipyridyl)dichlororuthenium(II) hexahydrate (Rubpy), is doped inside nanochannels of a silica matrix. The hydrophobic groups in the silica matrix avoid the leakage of dye from open channels. The amines groups on the surface of the FCMSN improve the modification performance of the nanoparticle. Because the nanochannels are isolated by a network skeleton of silica, fluorescence quenching based on the inner filter effect of the fluorescent dyes immobilized in nanochannels is weakened effectively. The Quantum Yield of obtained 90nm silica particles was about 61%. Compared with the fluorescent core–shell nanoparticle, the chemiluminescence reagents can freely enter the nanoparticles to react with fluorescent dyes to create chemiluminescence. The results show that the FCMSN are both fluorescent labels and chemiluminescent labels. In biological applications, the NaIO4oxidation method was proven to be superior to the glutaraldehyde method. The amount of amino could affect the specificity of the FCMSN. The fluorescence microscopy imaging demonstrated that the FCMSN is viable for biological applications.