Origin of periendothelial cells in microvessels derived from human microvascular endothelial cells.
Origin of periendothelial cells in microvessels derived from human microvascular endothelial cells.
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微血管中内皮细胞的起源源自人微血管内皮细胞。
DOI：
10.1016/j.biocel.2007.10.012
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Martins-Green,Manuela
中科院分区：
文献类型：
--
作者：
Njauw,Ching-Ni;Yuan,Hongwei;Zheng,Lei;Yao,Min;Martins-Green,Manuela
在微血管中，表达α -平滑肌肌动蛋白（αSMA）的内皮细胞与内皮细胞相互作用，对血管成熟和稳定至关重要。在成人组织中，内皮周围细胞的细胞起源仍然不清楚，特别是在人类中。为了确定人类内皮细胞的来源，我们使用了最近开发的3D共培养系统，模拟人类皮肤结缔组织。该系统由正常人真皮成纤维细胞（NHDF）、人真皮微血管内皮细胞（HMEC-1）和胶原基质组成。在这个系统中，“微血管”由内皮腔和内皮周围细胞组成。使用该共培养系统，我们(i)用活性染料CFDA-SE标记成纤维细胞，与未标记的内皮细胞一起培养，观察到只有内皮相关的CFDA-SE标记细胞表达αSMA；（ii）用稳定表达eGFP的逆转录病毒感染内皮细胞，与未标记的成纤维细胞一起培养，观察到表达α - sma的细胞不共表达eGFP，但与微血管内皮细胞表达eGFP相关。综上所述，这些结果表明，内皮周围细胞是由成纤维细胞分化而来的，它们需要与内皮细胞相互作用才能分化。
In microvessels, periendothelial cells expressing alpha smooth muscle actin (αSMA) interact with the endothelial cells and are essential for vessel maturation and stabilization. In adult tissues, the cellular origin of the periendothelial cells is still not clear, in particular in humans. To determine the origin of human periendothelial cells, we used a recently developed 3D co-culture system that mimics human skin connective tissue. This system is composed of normal human dermal fibroblasts (NHDF), human dermal microvascular endothelial cells (HMEC-1), and a collagen matrix. In this system, “microvessels” composed of an endothelial lumen with associated with periendothelial cells develop. Using this co-culture system, we (i) labelled fibroblasts with the vital dye CFDA-SE, cultured them with unlabelled endothelial cells, and observed that only endothelium-associated CFDA-SE-labelled cells express αSMA; (ii) infected endothelial cells with a retrovirus stably expressing eGFP, cultured them with unlabelled fibroblasts, and observed that cells expressing αSMA did not co-express eGFP, but were associated with the eGFP-expressing endothelial cells of the microvessels. Together, these results indicate that periendothelial cells arise by differentiation from fibroblasts and that they require interaction with endothelial cells to do so.