Bromodeoxyuridine amplifies free-radical-mediated DNA damage.
Bromodeoxyuridine amplifies free-radical-mediated DNA damage.
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溴脱氧尿苷会放大自由基介导的 DNA 损伤。
DOI：
10.1042/bj2610269
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Hoehn,H
中科院分区：
文献类型：
--
作者：
Poot,M;Rabinovitch,PS;Hoehn,H
升高的氧浓度和百草枯（一种产生超氧化物的化合物）诱导细胞停滞在细胞周期的G2期，这可以通过向培养基中添加溴脱氧尿苷（BrdU）来增强。亲脂性过氧化氢枯烯和自由基清除剂维生素E的实验表明，BrdU依赖的G2期阻滞不是由脂质过氧化介导的。G2期阻滞的BrdU依赖性可用作检测由氧自由基引起的DNA损伤的灵敏细胞生物学测定。
Elevated oxygen concentrations and paraquat, a superoxide-generating compound, induce an arrest of cells in the G2 phase of the cell cycle, which can be enhanced by adding bromodeoxyuridine (BrdU) to the culture medium. Experiments with the lipophilic peroxide cumene hydroperoxide and the free-radical scavenger vitamin E demonstrate that the BrdU-dependent G2 arrest is not mediated by lipid peroxidation. The BrdU-dependency of arrest in the G2 phase can be used as a sensitive cell biological assay to detect DNA damage elicited by oxygen free radicals.